Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3327

Experiment: Stearic 100 uM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3326 and Rv3327 are separated by 54 nucleotidesRv3327 and Rv3328c overlap by 68 nucleotidesRv3328c and Rv3329 are separated by 59 nucleotides Rv3325: Rv3325 - Probable transposase for insertion sequence element IS6110 (fragment), at 3,710,433 to 3,710,759 Rv3325 Rv3326: Rv3326 - Probable transposase, at 3,710,708 to 3,711,694 Rv3326 Rv3327: Rv3327 - Probable transposase fusion protein, at 3,711,749 to 3,713,461 Rv3327 Rv3328c: Rv3328c - Probable alternative RNA polymerase sigma factor (fragment) SigJ, at 3,713,394 to 3,714,332 Rv3328c Rv3329: Rv3329 - Probable aminotransferase, at 3,714,392 to 3,715,708 Rv3329 Position (kb) 3711 3712 3713 3714Strain fitness (log2 ratio) -1 0 1at 3712.096 kb on - strand, within Rv3327at 3712.096 kb on - strand, within Rv3327at 3712.807 kb on + strand, within Rv3327at 3712.814 kb on + strand, within Rv3327at 3712.848 kb on + strand, within Rv3327at 3712.856 kb on - strand, within Rv3327at 3712.856 kb on - strand, within Rv3327at 3712.884 kb on + strand, within Rv3327at 3713.180 kb on + strand, within Rv3327at 3713.181 kb on - strand, within Rv3327at 3713.327 kb on + strandat 3713.328 kb on - strandat 3713.659 kb on - strand, within Rv3328cat 3714.112 kb on + strand, within Rv3328cat 3714.112 kb on + strand, within Rv3328cat 3714.112 kb on + strand, within Rv3328cat 3714.113 kb on - strand, within Rv3328cat 3714.281 kb on - strandat 3714.352 kb on + strandat 3714.353 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Stearic 100 uM (C)
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3,712,096 - Rv3327 0.20 -0.0
3,712,096 - Rv3327 0.20 -0.0
3,712,807 + Rv3327 0.62 -0.3
3,712,814 + Rv3327 0.62 -0.8
3,712,848 + Rv3327 0.64 -1.5
3,712,856 - Rv3327 0.65 -0.2
3,712,856 - Rv3327 0.65 -0.6
3,712,884 + Rv3327 0.66 +0.7
3,713,180 + Rv3327 0.84 -0.4
3,713,181 - Rv3327 0.84 +0.0
3,713,327 + -0.2
3,713,328 - +0.1
3,713,659 - Rv3328c 0.28 -0.2
3,714,112 + Rv3328c 0.76 +0.6
3,714,112 + Rv3328c 0.76 +0.3
3,714,112 + Rv3328c 0.76 +1.1
3,714,113 - Rv3328c 0.77 -1.0
3,714,281 - -0.4
3,714,352 + -0.1
3,714,353 - +1.2

Or see this region's nucleotide sequence