Experiment: Stearic 100 uM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv2981c and Rv2982c are separated by 77 nucleotides Rv2982c and Rv2983 are separated by 118 nucleotides Rv2983 and Rv2984 are separated by 91 nucleotides
Rv2981c: Rv2981c - Probable D-alanine--D-alanine ligase DdlA (D-alanylalanine synthetase) (D-ala-D-ala ligase), at 3,336,796 to 3,337,917
Rv2981c
Rv2982c: Rv2982c - Probable glycerol-3-phosphate dehydrogenase [NAD(P)+] GpdA2 (NAD(P)H-dependent glycerol-3-phosphate dehydrogenase), at 3,337,995 to 3,338,999
Rv2982c
Rv2983: Rv2983 - Conserved hypothetical alanine rich protein, at 3,339,118 to 3,339,762
Rv2983
Rv2984: Rv2984 - Polyphosphate kinase PPK (polyphosphoric acid kinase) (ATP-polyphosphate phosphotransferase), at 3,339,854 to 3,342,082
Rv2984
Position (kb)
3337
3338
3339 Strain fitness (log2 ratio)
-1
0
1
2 at 3337.909 kb on - strand at 3338.057 kb on + strand at 3338.058 kb on - strand at 3338.104 kb on + strand, within Rv2982c at 3338.104 kb on + strand, within Rv2982c at 3338.461 kb on + strand, within Rv2982c at 3338.473 kb on + strand, within Rv2982c at 3338.823 kb on + strand, within Rv2982c at 3338.855 kb on - strand, within Rv2982c at 3338.865 kb on - strand, within Rv2982c at 3338.865 kb on - strand, within Rv2982c at 3338.988 kb on + strand at 3339.010 kb on + strand at 3339.725 kb on + strand at 3339.996 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Stearic 100 uM (C) remove 3,337,909 - -0.6 3,338,057 + -1.0 3,338,058 - +0.7 3,338,104 + Rv2982c 0.11 -1.0 3,338,104 + Rv2982c 0.11 +1.9 3,338,461 + Rv2982c 0.46 -1.6 3,338,473 + Rv2982c 0.48 -1.3 3,338,823 + Rv2982c 0.82 -1.4 3,338,855 - Rv2982c 0.86 -0.6 3,338,865 - Rv2982c 0.87 -1.0 3,338,865 - Rv2982c 0.87 -1.2 3,338,988 + -0.9 3,339,010 + -0.2 3,339,725 + -0.4 3,339,996 + -0.5
Or see this region's nucleotide sequence