Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0357c

Experiment: Stearic 100 uM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0355c and Rv0356c are separated by 150 nucleotidesRv0356c and Rv0357c overlap by 4 nucleotidesRv0357c and Rv0358 are separated by 90 nucleotidesRv0358 and Rv0359 are separated by 10 nucleotides Rv0355c: Rv0355c - PPE family protein PPE8, at 424,777 to 434,679 Rv0355c Rv0356c: Rv0356c - Conserved protein, at 434,830 to 435,474 Rv0356c Rv0357c: Rv0357c - Probable adenylosuccinate synthetase PurA (imp--aspartate ligase) (ADSS) (ampsase), at 435,471 to 436,769 Rv0357c Rv0358: Rv0358 - Conserved protein, at 436,860 to 437,507 Rv0358 Rv0359: Rv0359 - Probable conserved integral membrane protein, at 437,518 to 438,297 Rv0359 Position (kb) 435 436 437Strain fitness (log2 ratio) -1 0 1 2at 434.633 kb on + strandat 434.633 kb on + strandat 434.633 kb on + strandat 434.633 kb on + strandat 434.681 kb on + strandat 434.681 kb on + strandat 434.681 kb on + strandat 434.682 kb on - strandat 434.772 kb on + strandat 434.772 kb on + strandat 434.773 kb on - strandat 434.831 kb on + strandat 434.848 kb on + strandat 434.957 kb on - strand, within Rv0356cat 435.404 kb on + strand, within Rv0356cat 436.798 kb on - strandat 436.830 kb on - strandat 436.830 kb on - strandat 436.830 kb on - strandat 436.863 kb on + strandat 437.642 kb on - strand, within Rv0359at 437.759 kb on - strand, within Rv0359

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Per-strain Table

Position Strand Gene LocusTag Fraction Stearic 100 uM (C)
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434,633 + -0.1
434,633 + +0.2
434,633 + +0.2
434,633 + +1.9
434,681 + -0.6
434,681 + -0.2
434,681 + -0.9
434,682 - -0.3
434,772 + -0.4
434,772 + -1.2
434,773 - +0.2
434,831 + +0.1
434,848 + -0.1
434,957 - Rv0356c 0.20 +0.6
435,404 + Rv0356c 0.89 +0.9
436,798 - -0.6
436,830 - -0.3
436,830 - -0.1
436,830 - +1.3
436,863 + +0.3
437,642 - Rv0359 0.16 +0.4
437,759 - Rv0359 0.31 +0.6

Or see this region's nucleotide sequence