Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2249c

Experiment: butyrate 10 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2248 and Rv2249c are separated by 65 nucleotidesRv2249c and Rv2250c overlap by 7 nucleotidesRv2250c and Rv2250A are separated by 47 nucleotides Rv2248: Rv2248 - Conserved hypothetical protein, at 2,522,360 to 2,523,175 Rv2248 Rv2249c: Rv2249c - Probable glycerol-3-phosphate dehydrogenase GlpD1, at 2,523,241 to 2,524,791 Rv2249c Rv2250c: Rv2250c - Possible transcriptional regulatory protein, at 2,524,785 to 2,525,354 Rv2250c Rv2250A: Rv2250A - Possible flavoprotein, at 2,525,402 to 2,525,821 Rv2250A Rv2251: Rv2251 - Possible flavoprotein, at 2,525,565 to 2,526,992 Rv2251 Position (kb) 2523 2524 2525Strain fitness (log2 ratio) -1 0 1 2 3at 2522.304 kb on + strandat 2522.578 kb on - strand, within Rv2248at 2522.736 kb on + strand, within Rv2248at 2522.827 kb on - strand, within Rv2248at 2523.328 kb on - strandat 2523.620 kb on + strand, within Rv2249cat 2523.742 kb on + strand, within Rv2249cat 2523.762 kb on - strand, within Rv2249cat 2523.816 kb on + strand, within Rv2249cat 2524.560 kb on - strand, within Rv2249cat 2524.663 kb on + strandat 2524.795 kb on + strandat 2524.796 kb on - strandat 2524.796 kb on - strandat 2524.815 kb on - strandat 2525.020 kb on + strand, within Rv2250cat 2525.103 kb on + strand, within Rv2250cat 2525.594 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction butyrate 10 mM (C)
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2,522,304 + -0.4
2,522,578 - Rv2248 0.27 -0.8
2,522,736 + Rv2248 0.46 -0.1
2,522,827 - Rv2248 0.57 -0.1
2,523,328 - -0.2
2,523,620 + Rv2249c 0.24 -0.5
2,523,742 + Rv2249c 0.32 +0.1
2,523,762 - Rv2249c 0.34 +0.8
2,523,816 + Rv2249c 0.37 +0.4
2,524,560 - Rv2249c 0.85 +0.6
2,524,663 + +3.4
2,524,795 + -0.3
2,524,796 - +0.6
2,524,796 - +0.2
2,524,815 - -0.4
2,525,020 + Rv2250c 0.41 +0.1
2,525,103 + Rv2250c 0.56 +0.0
2,525,594 + -0.4

Or see this region's nucleotide sequence