Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2211c

Experiment: butyrate 10 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2210c and Rv2211c are separated by 71 nucleotidesRv2211c and Rv2212 are separated by 8 nucleotides Rv2210c: Rv2210c - Branched-chain amino acid transaminase IlvE, at 2,474,864 to 2,475,970 Rv2210c Rv2211c: Rv2211c - Probable aminomethyltransferase GcvT (glycine cleavage system T protein), at 2,476,042 to 2,477,181 Rv2211c Rv2212: Rv2212 - Adenylyl cyclase (ATP pyrophosphate-lyase) (adenylate cyclase), at 2,477,190 to 2,478,326 Rv2212 Position (kb) 2476 2477 2478Strain fitness (log2 ratio) -1 0 1at 2477.123 kb on - strandat 2477.183 kb on + strandat 2477.183 kb on + strandat 2477.184 kb on - strandat 2477.184 kb on - strandat 2477.184 kb on - strandat 2477.193 kb on + strandat 2477.268 kb on + strandat 2477.356 kb on + strand, within Rv2212at 2477.356 kb on + strand, within Rv2212at 2477.376 kb on + strand, within Rv2212at 2477.377 kb on - strand, within Rv2212at 2477.377 kb on - strand, within Rv2212at 2477.377 kb on - strand, within Rv2212at 2477.682 kb on + strand, within Rv2212at 2477.766 kb on + strand, within Rv2212at 2478.072 kb on + strand, within Rv2212at 2478.102 kb on + strand, within Rv2212at 2478.102 kb on + strand, within Rv2212

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Per-strain Table

Position Strand Gene LocusTag Fraction butyrate 10 mM (C)
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2,477,123 - -1.4
2,477,183 + +0.0
2,477,183 + +0.1
2,477,184 - -0.4
2,477,184 - -0.8
2,477,184 - -0.6
2,477,193 + -0.9
2,477,268 + -0.2
2,477,356 + Rv2212 0.15 -0.5
2,477,356 + Rv2212 0.15 -0.9
2,477,376 + Rv2212 0.16 +0.9
2,477,377 - Rv2212 0.16 -0.6
2,477,377 - Rv2212 0.16 -0.8
2,477,377 - Rv2212 0.16 +0.4
2,477,682 + Rv2212 0.43 -0.6
2,477,766 + Rv2212 0.51 +0.7
2,478,072 + Rv2212 0.78 +0.4
2,478,102 + Rv2212 0.80 +0.2
2,478,102 + Rv2212 0.80 -0.2

Or see this region's nucleotide sequence