Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2226

Experiment: D-Trehalose 10 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2225 and Rv2226 are separated by 244 nucleotidesRv2226 and Rv2227 are separated by 557 nucleotides Rv2225: Rv2225 - 3-methyl-2-oxobutanoate hydroxymethyltransferase PanB, at 2,497,742 to 2,498,587 Rv2225 Rv2226: Rv2226 - Conserved protein, at 2,498,832 to 2,500,373 Rv2226 Rv2227: Rv2227 - Conserved hypothetical protein, at 2,500,931 to 2,501,632 Rv2227 Position (kb) 2498 2499 2500 2501Strain fitness (log2 ratio) -2 -1 0 1 2at 2498.772 kb on + strandat 2498.772 kb on + strandat 2498.772 kb on + strandat 2498.773 kb on - strandat 2498.773 kb on - strandat 2498.783 kb on - strandat 2498.840 kb on - strandat 2499.603 kb on + strand, within Rv2226at 2499.604 kb on - strand, within Rv2226at 2499.903 kb on + strand, within Rv2226at 2500.267 kb on - strandat 2500.267 kb on - strandat 2500.267 kb on - strandat 2500.267 kb on - strandat 2500.267 kb on - strandat 2500.831 kb on + strandat 2500.880 kb on + strandat 2500.989 kb on + strandat 2501.053 kb on - strand, within Rv2227at 2501.087 kb on + strand, within Rv2227at 2501.338 kb on - strand, within Rv2227

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Trehalose 10 mM (C)
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2,498,772 + -0.1
2,498,772 + -0.5
2,498,772 + -0.2
2,498,773 - +0.2
2,498,773 - -0.6
2,498,783 - +0.4
2,498,840 - -0.3
2,499,603 + Rv2226 0.50 -0.9
2,499,604 - Rv2226 0.50 -0.9
2,499,903 + Rv2226 0.69 -2.0
2,500,267 - -0.4
2,500,267 - +0.5
2,500,267 - -2.2
2,500,267 - -0.4
2,500,267 - +0.2
2,500,831 + +1.9
2,500,880 + -0.1
2,500,989 + -0.4
2,501,053 - Rv2227 0.17 -0.8
2,501,087 + Rv2227 0.22 +0.0
2,501,338 - Rv2227 0.58 -0.3

Or see this region's nucleotide sequence