Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv1327c

Experiment: D-Trehalose 10 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv1326c and Rv1327c are separated by 7 nucleotidesRv1327c and Rv1328 are separated by 138 nucleotides Rv1326c: Rv1326c - 1,4-alpha-glucan branching enzyme GlgB (glycogen branching enzyme), at 1,490,117 to 1,492,312 Rv1326c Rv1327c: Rv1327c - Probable glucanase GlgE, at 1,492,320 to 1,494,425 Rv1327c Rv1328: Rv1328 - Probable glycogen phosphorylase GlgP, at 1,494,564 to 1,497,155 Rv1328 Position (kb) 1492 1493 1494 1495Strain fitness (log2 ratio) -3 -2 -1 0 1at 1494.439 kb on - strandat 1494.439 kb on - strandat 1494.483 kb on + strandat 1494.483 kb on + strandat 1494.484 kb on - strandat 1494.484 kb on - strandat 1494.487 kb on - strandat 1494.487 kb on - strandat 1494.550 kb on + strandat 1494.550 kb on + strandat 1494.555 kb on + strandat 1494.556 kb on - strandat 1494.635 kb on + strandat 1494.829 kb on - strand, within Rv1328at 1494.907 kb on - strand, within Rv1328at 1494.945 kb on - strand, within Rv1328at 1495.032 kb on + strand, within Rv1328at 1495.092 kb on + strand, within Rv1328at 1495.093 kb on - strand, within Rv1328at 1495.374 kb on + strand, within Rv1328at 1495.375 kb on - strand, within Rv1328at 1495.375 kb on - strand, within Rv1328

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Trehalose 10 mM (C)
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1,494,439 - -0.3
1,494,439 - -0.4
1,494,483 + -1.0
1,494,483 + -1.0
1,494,484 - -1.2
1,494,484 - -0.8
1,494,487 - +0.1
1,494,487 - -1.6
1,494,550 + -0.4
1,494,550 + -0.1
1,494,555 + -0.6
1,494,556 - +0.1
1,494,635 + -0.8
1,494,829 - Rv1328 0.10 -1.7
1,494,907 - Rv1328 0.13 -0.6
1,494,945 - Rv1328 0.15 -0.1
1,495,032 + Rv1328 0.18 -0.8
1,495,092 + Rv1328 0.20 -0.2
1,495,093 - Rv1328 0.20 -1.0
1,495,374 + Rv1328 0.31 -1.9
1,495,375 - Rv1328 0.31 -2.8
1,495,375 - Rv1328 0.31 -1.0

Or see this region's nucleotide sequence