Experiment: L-Lactate 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3011c and Rv3012c overlap by 4 nucleotides Rv3012c and Rv3013 are separated by 84 nucleotides Rv3013 and Rv3014c are separated by 73 nucleotides
Rv3011c: Rv3011c - Probable glutamyl-tRNA(GLN) amidotransferase (subunit A) GatA (Glu-ADT subunit A), at 3,369,950 to 3,371,434
Rv3011c
Rv3012c: Rv3012c - Probable glutamyl-tRNA(GLN) amidotransferase (subunit C) GatC (Glu-ADT subunit C), at 3,371,431 to 3,371,730
Rv3012c
Rv3013: Rv3013 - Conserved protein, at 3,371,815 to 3,372,471
Rv3013
Rv3014c: Rv3014c - DNA ligase [NAD dependent] LigA (polydeoxyribonucleotide synthase [NAD+]), at 3,372,545 to 3,374,620
Rv3014c
Position (kb)
3371
3372
3373 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3371.743 kb on + strand at 3371.789 kb on + strand at 3371.790 kb on - strand at 3371.790 kb on - strand at 3371.824 kb on + strand at 3371.824 kb on + strand at 3371.824 kb on + strand at 3371.824 kb on + strand at 3371.825 kb on - strand at 3371.825 kb on - strand at 3371.828 kb on + strand at 3371.829 kb on - strand at 3372.336 kb on + strand, within Rv3013 at 3372.437 kb on - strand at 3372.437 kb on - strand at 3372.441 kb on - strand at 3372.505 kb on - strand at 3372.546 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lactate 10 mM (C) remove 3,371,743 + -0.4 3,371,789 + -0.3 3,371,790 - -1.8 3,371,790 - +0.1 3,371,824 + -0.5 3,371,824 + +0.3 3,371,824 + -0.0 3,371,824 + -0.4 3,371,825 - +0.7 3,371,825 - -0.3 3,371,828 + -0.7 3,371,829 - +0.0 3,372,336 + Rv3013 0.79 -0.7 3,372,437 - +3.0 3,372,437 - -0.2 3,372,441 - -1.5 3,372,505 - +0.3 3,372,546 + -1.0
Or see this region's nucleotide sequence