Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3013

Experiment: L-Lactate 10 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3011c and Rv3012c overlap by 4 nucleotidesRv3012c and Rv3013 are separated by 84 nucleotidesRv3013 and Rv3014c are separated by 73 nucleotides Rv3011c: Rv3011c - Probable glutamyl-tRNA(GLN) amidotransferase (subunit A) GatA (Glu-ADT subunit A), at 3,369,950 to 3,371,434 Rv3011c Rv3012c: Rv3012c - Probable glutamyl-tRNA(GLN) amidotransferase (subunit C) GatC (Glu-ADT subunit C), at 3,371,431 to 3,371,730 Rv3012c Rv3013: Rv3013 - Conserved protein, at 3,371,815 to 3,372,471 Rv3013 Rv3014c: Rv3014c - DNA ligase [NAD dependent] LigA (polydeoxyribonucleotide synthase [NAD+]), at 3,372,545 to 3,374,620 Rv3014c Position (kb) 3371 3372 3373Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3371.743 kb on + strandat 3371.789 kb on + strandat 3371.790 kb on - strandat 3371.790 kb on - strandat 3371.824 kb on + strandat 3371.824 kb on + strandat 3371.824 kb on + strandat 3371.824 kb on + strandat 3371.825 kb on - strandat 3371.825 kb on - strandat 3371.828 kb on + strandat 3371.829 kb on - strandat 3372.336 kb on + strand, within Rv3013at 3372.437 kb on - strandat 3372.437 kb on - strandat 3372.441 kb on - strandat 3372.505 kb on - strandat 3372.546 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Lactate 10 mM (C)
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3,371,743 + -0.4
3,371,789 + -0.3
3,371,790 - -1.8
3,371,790 - +0.1
3,371,824 + -0.5
3,371,824 + +0.3
3,371,824 + -0.0
3,371,824 + -0.4
3,371,825 - +0.7
3,371,825 - -0.3
3,371,828 + -0.7
3,371,829 - +0.0
3,372,336 + Rv3013 0.79 -0.7
3,372,437 - +3.0
3,372,437 - -0.2
3,372,441 - -1.5
3,372,505 - +0.3
3,372,546 + -1.0

Or see this region's nucleotide sequence