Experiment: L-Lactate 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0663 and Rv0664 are separated by 31 nucleotides Rv0664 and Rv0665 overlap by 4 nucleotides Rv0665 and Rv0666 overlap by 4 nucleotides Rv0666 and Rv0667 are separated by 497 nucleotides
Rv0663: Rv0663 - Possible arylsulfatase AtsD (aryl-sulfate sulphohydrolase) (arylsulphatase), at 756,137 to 758,500
Rv0663
Rv0664: Rv0664 - Possible antitoxin VapB8, at 758,532 to 758,804
Rv0664
Rv0665: Rv0665 - Possible toxin VapC8, at 758,801 to 759,139
Rv0665
Rv0666: Rv0666 - Possible membrane protein, at 759,136 to 759,309
Rv0666
Rv0667: Rv0667 - DNA-directed RNA polymerase (beta chain) RpoB (transcriptase beta chain) (RNA polymerase beta subunit), at 759,807 to 763,325
Rv0667
Position (kb)
758
759
760 Strain fitness (log2 ratio)
-1
0
1 at 758.117 kb on + strand, within Rv0663 at 758.123 kb on + strand, within Rv0663 at 758.151 kb on - strand, within Rv0663 at 758.787 kb on - strand at 758.832 kb on + strand at 758.837 kb on - strand, within Rv0665 at 758.908 kb on + strand, within Rv0665 at 759.410 kb on + strand at 759.545 kb on + strand at 759.555 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lactate 10 mM (C) remove 758,117 + Rv0663 0.84 -0.9 758,123 + Rv0663 0.84 +0.2 758,151 - Rv0663 0.85 -0.2 758,787 - -0.7 758,832 + -0.0 758,837 - Rv0665 0.11 -0.8 758,908 + Rv0665 0.32 -0.2 759,410 + -1.0 759,545 + +0.2 759,555 + -0.8
Or see this region's nucleotide sequence