Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0665

Experiment: L-Lactate 10 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0663 and Rv0664 are separated by 31 nucleotidesRv0664 and Rv0665 overlap by 4 nucleotidesRv0665 and Rv0666 overlap by 4 nucleotidesRv0666 and Rv0667 are separated by 497 nucleotides Rv0663: Rv0663 - Possible arylsulfatase AtsD (aryl-sulfate sulphohydrolase) (arylsulphatase), at 756,137 to 758,500 Rv0663 Rv0664: Rv0664 - Possible antitoxin VapB8, at 758,532 to 758,804 Rv0664 Rv0665: Rv0665 - Possible toxin VapC8, at 758,801 to 759,139 Rv0665 Rv0666: Rv0666 - Possible membrane protein, at 759,136 to 759,309 Rv0666 Rv0667: Rv0667 - DNA-directed RNA polymerase (beta chain) RpoB (transcriptase beta chain) (RNA polymerase beta subunit), at 759,807 to 763,325 Rv0667 Position (kb) 758 759 760Strain fitness (log2 ratio) -1 0 1at 758.117 kb on + strand, within Rv0663at 758.123 kb on + strand, within Rv0663at 758.151 kb on - strand, within Rv0663at 758.787 kb on - strandat 758.832 kb on + strandat 758.837 kb on - strand, within Rv0665at 758.908 kb on + strand, within Rv0665at 759.410 kb on + strandat 759.545 kb on + strandat 759.555 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Lactate 10 mM (C)
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758,117 + Rv0663 0.84 -0.9
758,123 + Rv0663 0.84 +0.2
758,151 - Rv0663 0.85 -0.2
758,787 - -0.7
758,832 + -0.0
758,837 - Rv0665 0.11 -0.8
758,908 + Rv0665 0.32 -0.2
759,410 + -1.0
759,545 + +0.2
759,555 + -0.8

Or see this region's nucleotide sequence