Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0557

Experiment: L-Lactate 10 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0555 and Rv0556 overlap by 4 nucleotidesRv0556 and Rv0557 are separated by 61 nucleotidesRv0557 and Rv0558 are separated by 16 nucleotidesRv0558 and Rv0559c are separated by 13 nucleotides Rv0555: Rv0555 - Probable bifunctional menaquinone biosynthesis protein MenD : 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (SHCHC synthase) + 2-oxoglutarate decarboxylase (alpha-ketoglutarate decarboxylase) (KDC), at 646,298 to 647,962 Rv0555 Rv0556: Rv0556 - Probable conserved transmembrane protein, at 647,959 to 648,474 Rv0556 Rv0557: Rv0557 - Mannosyltransferase MgtA, at 648,536 to 649,672 Rv0557 Rv0558: Rv0558 - Probable ubiquinone/menaquinone biosynthesis methyltransferase MenH (2-heptaprenyl-1,4-naphthoquinone methyltransferase), at 649,689 to 650,393 Rv0558 Rv0559c: Rv0559c - Possible conserved secreted protein, at 650,407 to 650,745 Rv0559c Position (kb) 648 649 650Strain fitness (log2 ratio) -1 0 1at 648.417 kb on - strand, within Rv0556at 648.496 kb on - strandat 648.496 kb on - strandat 649.464 kb on + strand, within Rv0557at 649.632 kb on - strandat 649.688 kb on + strandat 650.454 kb on - strand, within Rv0559cat 650.543 kb on + strand, within Rv0559cat 650.543 kb on + strand, within Rv0559cat 650.543 kb on + strand, within Rv0559cat 650.544 kb on - strand, within Rv0559cat 650.607 kb on - strand, within Rv0559cat 650.621 kb on + strand, within Rv0559c

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Lactate 10 mM (C)
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648,417 - Rv0556 0.89 -0.1
648,496 - +0.3
648,496 - -0.4
649,464 + Rv0557 0.82 -0.6
649,632 - -0.1
649,688 + -1.2
650,454 - Rv0559c 0.14 -0.3
650,543 + Rv0559c 0.40 -0.5
650,543 + Rv0559c 0.40 -0.2
650,543 + Rv0559c 0.40 +0.6
650,544 - Rv0559c 0.40 +0.6
650,607 - Rv0559c 0.59 -1.0
650,621 + Rv0559c 0.63 -0.5

Or see this region's nucleotide sequence