Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3837c

Experiment: 7H9 with Hygromycin B 0.0095 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3835 and Rv3836 are separated by 4 nucleotidesRv3836 and Rv3837c are separated by 194 nucleotidesRv3837c and Rv3838c overlap by 4 nucleotides Rv3835: Rv3835 - Conserved membrane protein, at 4,309,047 to 4,310,396 Rv3835 Rv3836: Rv3836 - Conserved hypothetical protein, at 4,310,401 to 4,310,814 Rv3836 Rv3837c: Rv3837c - Probable phosphoglycerate mutase (phosphoglyceromutase) (phosphoglycerate phosphomutase), at 4,311,009 to 4,311,707 Rv3837c Rv3838c: Rv3838c - Prephenate dehydratase PheA, at 4,311,704 to 4,312,669 Rv3838c Position (kb) 4311 4312Strain fitness (log2 ratio) -1 0 1 2 3at 4310.201 kb on + strand, within Rv3835at 4310.394 kb on + strandat 4310.504 kb on + strand, within Rv3836at 4310.505 kb on - strand, within Rv3836at 4310.549 kb on - strand, within Rv3836at 4310.584 kb on + strand, within Rv3836at 4310.618 kb on - strand, within Rv3836at 4310.799 kb on + strandat 4310.799 kb on + strandat 4310.799 kb on + strandat 4310.800 kb on - strandat 4310.936 kb on + strandat 4310.948 kb on - strandat 4310.948 kb on - strandat 4311.320 kb on - strand, within Rv3837cat 4311.320 kb on - strand, within Rv3837cat 4311.394 kb on + strand, within Rv3837cat 4311.395 kb on - strand, within Rv3837cat 4311.395 kb on - strand, within Rv3837cat 4311.395 kb on - strand, within Rv3837cat 4311.395 kb on - strand, within Rv3837cat 4311.395 kb on - strand, within Rv3837cat 4311.395 kb on - strand, within Rv3837cat 4311.450 kb on + strand, within Rv3837cat 4311.450 kb on + strand, within Rv3837cat 4311.450 kb on + strand, within Rv3837cat 4311.450 kb on + strand, within Rv3837cat 4311.450 kb on + strand, within Rv3837cat 4311.457 kb on - strand, within Rv3837cat 4311.667 kb on + strandat 4311.667 kb on + strandat 4311.667 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Hygromycin B 0.0095 mM
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4,310,201 + Rv3835 0.85 +0.1
4,310,394 + -0.2
4,310,504 + Rv3836 0.25 +0.1
4,310,505 - Rv3836 0.25 -0.5
4,310,549 - Rv3836 0.36 -1.2
4,310,584 + Rv3836 0.44 -0.5
4,310,618 - Rv3836 0.52 +0.4
4,310,799 + -0.1
4,310,799 + +0.5
4,310,799 + -0.3
4,310,800 - +0.0
4,310,936 + +2.8
4,310,948 - -0.7
4,310,948 - +0.7
4,311,320 - Rv3837c 0.44 -0.3
4,311,320 - Rv3837c 0.44 -0.0
4,311,394 + Rv3837c 0.55 -0.4
4,311,395 - Rv3837c 0.55 -0.5
4,311,395 - Rv3837c 0.55 -0.0
4,311,395 - Rv3837c 0.55 -0.1
4,311,395 - Rv3837c 0.55 +0.9
4,311,395 - Rv3837c 0.55 +1.2
4,311,395 - Rv3837c 0.55 +1.8
4,311,450 + Rv3837c 0.63 -0.3
4,311,450 + Rv3837c 0.63 -0.0
4,311,450 + Rv3837c 0.63 -0.1
4,311,450 + Rv3837c 0.63 -0.3
4,311,450 + Rv3837c 0.63 -0.1
4,311,457 - Rv3837c 0.64 -1.2
4,311,667 + -0.1
4,311,667 + -0.1
4,311,667 + +0.8

Or see this region's nucleotide sequence