Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3116

Experiment: 7H9 with Hygromycin B 0.0095 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3115 and Rv3116 are separated by 77 nucleotidesRv3116 and Rv3117 are separated by 28 nucleotidesRv3117 and Rv3118 are separated by 1 nucleotides Rv3115: Rv3115 - Probable transposase, at 3,481,451 to 3,482,698 Rv3115 Rv3116: Rv3116 - Probable molybdenum cofactor biosynthesis protein MoeB2 (MPT-synthase sulfurylase) (molybdopterin synthase sulphurylase), at 3,482,776 to 3,483,945 Rv3116 Rv3117: Rv3117 - Probable thiosulfate sulfurtransferase CysA3 (rhodanese-like protein) (thiosulfate cyanide transsulfurase) (thiosulfate thiotransferase), at 3,483,974 to 3,484,807 Rv3117 Rv3118: Rv3118 - Conserved hypothetical protein SseC1, at 3,484,809 to 3,485,111 Rv3118 Position (kb) 3482 3483 3484Strain fitness (log2 ratio) -1 0 1at 3482.811 kb on - strandat 3482.839 kb on + strandat 3482.907 kb on - strand, within Rv3116at 3482.913 kb on + strand, within Rv3116at 3482.973 kb on + strand, within Rv3116at 3483.054 kb on + strand, within Rv3116at 3483.268 kb on + strand, within Rv3116at 3483.422 kb on + strand, within Rv3116at 3483.485 kb on - strand, within Rv3116at 3483.505 kb on + strand, within Rv3116at 3483.506 kb on - strand, within Rv3116at 3483.627 kb on - strand, within Rv3116at 3483.627 kb on - strand, within Rv3116at 3483.627 kb on - strand, within Rv3116at 3483.941 kb on - strandat 3483.943 kb on + strandat 3483.943 kb on + strandat 3483.944 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Hygromycin B 0.0095 mM
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3,482,811 - -0.6
3,482,839 + -0.3
3,482,907 - Rv3116 0.11 -0.9
3,482,913 + Rv3116 0.12 -1.3
3,482,973 + Rv3116 0.17 -0.4
3,483,054 + Rv3116 0.24 +0.0
3,483,268 + Rv3116 0.42 -0.2
3,483,422 + Rv3116 0.55 -0.6
3,483,485 - Rv3116 0.61 +0.1
3,483,505 + Rv3116 0.62 -0.4
3,483,506 - Rv3116 0.62 -0.8
3,483,627 - Rv3116 0.73 -0.8
3,483,627 - Rv3116 0.73 +0.5
3,483,627 - Rv3116 0.73 -0.8
3,483,941 - -1.1
3,483,943 + -0.1
3,483,943 + -0.3
3,483,944 - +0.4

Or see this region's nucleotide sequence