Experiment: 7H9 with Hygromycin B 0.0095 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3115 and Rv3116 are separated by 77 nucleotides Rv3116 and Rv3117 are separated by 28 nucleotides Rv3117 and Rv3118 are separated by 1 nucleotides
Rv3115: Rv3115 - Probable transposase, at 3,481,451 to 3,482,698
Rv3115
Rv3116: Rv3116 - Probable molybdenum cofactor biosynthesis protein MoeB2 (MPT-synthase sulfurylase) (molybdopterin synthase sulphurylase), at 3,482,776 to 3,483,945
Rv3116
Rv3117: Rv3117 - Probable thiosulfate sulfurtransferase CysA3 (rhodanese-like protein) (thiosulfate cyanide transsulfurase) (thiosulfate thiotransferase), at 3,483,974 to 3,484,807
Rv3117
Rv3118: Rv3118 - Conserved hypothetical protein SseC1, at 3,484,809 to 3,485,111
Rv3118
Position (kb)
3482
3483
3484 Strain fitness (log2 ratio)
-1
0
1 at 3482.811 kb on - strand at 3482.839 kb on + strand at 3482.907 kb on - strand, within Rv3116 at 3482.913 kb on + strand, within Rv3116 at 3482.973 kb on + strand, within Rv3116 at 3483.054 kb on + strand, within Rv3116 at 3483.268 kb on + strand, within Rv3116 at 3483.422 kb on + strand, within Rv3116 at 3483.485 kb on - strand, within Rv3116 at 3483.505 kb on + strand, within Rv3116 at 3483.506 kb on - strand, within Rv3116 at 3483.627 kb on - strand, within Rv3116 at 3483.627 kb on - strand, within Rv3116 at 3483.627 kb on - strand, within Rv3116 at 3483.941 kb on - strand at 3483.943 kb on + strand at 3483.943 kb on + strand at 3483.944 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Hygromycin B 0.0095 mM remove 3,482,811 - -0.6 3,482,839 + -0.3 3,482,907 - Rv3116 0.11 -0.9 3,482,913 + Rv3116 0.12 -1.3 3,482,973 + Rv3116 0.17 -0.4 3,483,054 + Rv3116 0.24 +0.0 3,483,268 + Rv3116 0.42 -0.2 3,483,422 + Rv3116 0.55 -0.6 3,483,485 - Rv3116 0.61 +0.1 3,483,505 + Rv3116 0.62 -0.4 3,483,506 - Rv3116 0.62 -0.8 3,483,627 - Rv3116 0.73 -0.8 3,483,627 - Rv3116 0.73 +0.5 3,483,627 - Rv3116 0.73 -0.8 3,483,941 - -1.1 3,483,943 + -0.1 3,483,943 + -0.3 3,483,944 - +0.4
Or see this region's nucleotide sequence