Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv1831

Experiment: 7H9 with Hygromycin B 0.0095 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv1830 and Rv1831 are separated by 52 nucleotidesRv1831 and Rv1832 are separated by 48 nucleotides Rv1830: Rv1830 - Conserved hypothetical protein, at 2,074,841 to 2,075,518 Rv1830 Rv1831: Rv1831 - Hypothetical protein, at 2,075,571 to 2,075,828 Rv1831 Rv1832: Rv1832 - Probable glycine dehydrogenase GcvB (glycine decarboxylase) (glycine cleavage system P-protein), at 2,075,877 to 2,078,702 Rv1832 Position (kb) 2075 2076Strain fitness (log2 ratio) -2 -1 0 1 2at 2074.644 kb on - strandat 2074.719 kb on - strandat 2074.739 kb on + strandat 2074.762 kb on + strandat 2074.793 kb on + strandat 2074.793 kb on + strandat 2074.794 kb on - strandat 2074.794 kb on - strandat 2074.802 kb on + strandat 2074.820 kb on + strandat 2074.820 kb on + strandat 2074.851 kb on + strandat 2075.597 kb on - strand, within Rv1831at 2075.647 kb on + strand, within Rv1831at 2075.667 kb on + strand, within Rv1831at 2075.667 kb on + strand, within Rv1831at 2075.667 kb on + strand, within Rv1831at 2075.667 kb on + strand, within Rv1831at 2075.667 kb on + strand, within Rv1831at 2075.667 kb on + strand, within Rv1831at 2075.667 kb on + strand, within Rv1831at 2075.709 kb on + strand, within Rv1831at 2075.709 kb on + strand, within Rv1831at 2075.849 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Hygromycin B 0.0095 mM
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2,074,644 - +1.1
2,074,719 - -1.9
2,074,739 + +0.3
2,074,762 + -0.9
2,074,793 + -0.5
2,074,793 + -0.5
2,074,794 - -2.0
2,074,794 - -1.0
2,074,802 + -0.4
2,074,820 + -0.8
2,074,820 + +0.4
2,074,851 + -1.1
2,075,597 - Rv1831 0.10 -0.6
2,075,647 + Rv1831 0.29 -1.8
2,075,667 + Rv1831 0.37 +0.6
2,075,667 + Rv1831 0.37 -0.2
2,075,667 + Rv1831 0.37 -0.9
2,075,667 + Rv1831 0.37 +0.5
2,075,667 + Rv1831 0.37 -0.6
2,075,667 + Rv1831 0.37 -1.8
2,075,667 + Rv1831 0.37 +1.9
2,075,709 + Rv1831 0.53 -1.4
2,075,709 + Rv1831 0.53 +0.2
2,075,849 + -0.1

Or see this region's nucleotide sequence