Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv1252c

Experiment: 7H9 with Hygromycin B 0.0095 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv1251c and Rv1252c are separated by 55 nucleotidesRv1252c and Rv1253 are separated by 65 nucleotides Rv1251c: Rv1251c - Conserved hypothetical protein, at 1,395,821 to 1,399,240 Rv1251c Rv1252c: Rv1252c - Probable lipoprotein LprE, at 1,399,296 to 1,399,904 Rv1252c Rv1253: Rv1253 - Probable cold-shock DeaD-box protein A homolog DeaD (ATP-dependent RNA helicase dead homolog), at 1,399,970 to 1,401,661 Rv1253 Position (kb) 1399 1400Strain fitness (log2 ratio) -2 -1 0 1at 1398.543 kb on - strand, within Rv1251cat 1398.810 kb on - strand, within Rv1251cat 1398.930 kb on + strandat 1399.174 kb on - strandat 1399.212 kb on + strandat 1399.213 kb on - strandat 1399.384 kb on + strand, within Rv1252cat 1399.445 kb on + strand, within Rv1252cat 1399.468 kb on + strand, within Rv1252cat 1399.469 kb on - strand, within Rv1252cat 1399.564 kb on + strand, within Rv1252cat 1399.793 kb on + strand, within Rv1252cat 1399.908 kb on - strandat 1399.985 kb on + strandat 1400.289 kb on - strand, within Rv1253at 1400.318 kb on + strand, within Rv1253at 1400.319 kb on - strand, within Rv1253at 1400.396 kb on - strand, within Rv1253at 1400.400 kb on + strand, within Rv1253at 1400.400 kb on + strand, within Rv1253at 1400.400 kb on + strand, within Rv1253at 1400.400 kb on + strand, within Rv1253at 1400.401 kb on - strand, within Rv1253at 1400.401 kb on - strand, within Rv1253at 1400.401 kb on - strand, within Rv1253at 1400.528 kb on + strand, within Rv1253

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Hygromycin B 0.0095 mM
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1,398,543 - Rv1251c 0.80 +0.4
1,398,810 - Rv1251c 0.87 -0.5
1,398,930 + -0.5
1,399,174 - -0.1
1,399,212 + -0.7
1,399,213 - +0.1
1,399,384 + Rv1252c 0.14 -0.2
1,399,445 + Rv1252c 0.24 -0.3
1,399,468 + Rv1252c 0.28 -1.0
1,399,469 - Rv1252c 0.28 -0.5
1,399,564 + Rv1252c 0.44 -0.3
1,399,793 + Rv1252c 0.82 -0.5
1,399,908 - -0.3
1,399,985 + -0.1
1,400,289 - Rv1253 0.19 -0.1
1,400,318 + Rv1253 0.21 -0.0
1,400,319 - Rv1253 0.21 -2.7
1,400,396 - Rv1253 0.25 +0.4
1,400,400 + Rv1253 0.25 -0.2
1,400,400 + Rv1253 0.25 +0.1
1,400,400 + Rv1253 0.25 +0.2
1,400,400 + Rv1253 0.25 -0.9
1,400,401 - Rv1253 0.25 +0.1
1,400,401 - Rv1253 0.25 +0.2
1,400,401 - Rv1253 0.25 -0.4
1,400,528 + Rv1253 0.33 -0.3

Or see this region's nucleotide sequence