Experiment: 7H9 with Hygromycin B 0.0095 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0728c and Rv0729 are separated by 30 nucleotides Rv0729 and Rv0730 are separated by 12 nucleotides Rv0730 and Rv0731c are separated by 88 nucleotides
Rv0728c: Rv0728c - Possible D-3-phosphoglycerate dehydrogenase SerA2 (phosphoglycerate dehydrogenase) (PGDH), at 820,496 to 821,476
Rv0728c
Rv0729: Rv0729 - Possible D-xylulose kinase XylB (xylulokinase) (xylulose kinase), at 821,507 to 822,853
Rv0729
Rv0730: Rv0730 - GCN5-related N-acetyltransferase, at 822,866 to 823,594
Rv0730
Rv0731c: Rv0731c - Possible S-adenosylmethionine-dependent methyltransferase, at 823,683 to 824,639
Rv0731c
Position (kb) 821
822
823 Strain fitness (log2 ratio) -2
-1
0
1
2 at 820.508 kb on + strand at 820.508 kb on + strand at 820.509 kb on - strand at 821.356 kb on - strand, within Rv0728c at 821.391 kb on + strand at 821.393 kb on + strand at 821.393 kb on + strand at 821.394 kb on - strand at 821.949 kb on - strand, within Rv0729 at 821.978 kb on + strand, within Rv0729 at 821.979 kb on - strand, within Rv0729 at 821.979 kb on - strand, within Rv0729 at 822.205 kb on - strand, within Rv0729 at 822.356 kb on - strand, within Rv0729 at 822.415 kb on + strand, within Rv0729 at 822.415 kb on + strand, within Rv0729 at 822.416 kb on - strand, within Rv0729 at 822.416 kb on - strand, within Rv0729 at 822.417 kb on + strand, within Rv0729 at 822.417 kb on + strand, within Rv0729 at 822.417 kb on + strand, within Rv0729 at 822.417 kb on + strand, within Rv0729 at 822.417 kb on + strand, within Rv0729 at 822.418 kb on - strand, within Rv0729 at 822.418 kb on - strand, within Rv0729 at 822.896 kb on + strand at 822.896 kb on + strand at 822.896 kb on + strand at 822.896 kb on + strand at 822.897 kb on - strand at 823.592 kb on + strand at 823.681 kb on - strand at 823.681 kb on - strand at 823.726 kb on + strand at 823.726 kb on + strand at 823.727 kb on - strand at 823.837 kb on + strand, within Rv0731c at 823.838 kb on - strand, within Rv0731c at 823.838 kb on - strand, within Rv0731c
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Hygromycin B 0.0095 mM remove 820,508 + -2.6 820,508 + -0.3 820,509 - +0.1 821,356 - Rv0728c 0.88 +0.5 821,391 + -0.9 821,393 + -0.1 821,393 + -0.4 821,394 - -0.9 821,949 - Rv0729 0.33 +0.2 821,978 + Rv0729 0.35 +0.8 821,979 - Rv0729 0.35 -2.5 821,979 - Rv0729 0.35 -0.2 822,205 - Rv0729 0.52 -0.4 822,356 - Rv0729 0.63 -0.5 822,415 + Rv0729 0.67 -0.5 822,415 + Rv0729 0.67 -0.5 822,416 - Rv0729 0.67 +1.8 822,416 - Rv0729 0.67 -0.3 822,417 + Rv0729 0.68 +1.2 822,417 + Rv0729 0.68 -0.7 822,417 + Rv0729 0.68 -0.1 822,417 + Rv0729 0.68 -0.9 822,417 + Rv0729 0.68 -0.3 822,418 - Rv0729 0.68 -0.5 822,418 - Rv0729 0.68 +0.3 822,896 + -0.5 822,896 + -0.7 822,896 + +0.5 822,896 + -0.9 822,897 - +0.2 823,592 + -0.9 823,681 - -0.7 823,681 - -0.2 823,726 + +0.7 823,726 + -0.3 823,727 - -0.5 823,837 + Rv0731c 0.16 +0.8 823,838 - Rv0731c 0.16 -0.7 823,838 - Rv0731c 0.16 -0.2
Or see this region's nucleotide sequence