Experiment: 7H9 with Hygromycin B 0.0095 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3001c and Rv3002c are separated by 37 nucleotides Rv3002c and Rv3003c overlap by 1 nucleotides Rv3003c and Rv3004 are separated by 361 nucleotides Rv3004 and Rv3005c are separated by 6 nucleotides
Rv3001c: Rv3001c - Probable KETOL-acid reductoisomerase IlvC (acetohydroxy-acid isomeroreductase) (alpha-keto-beta-hydroxylacil reductoisomerase), at 3,359,585 to 3,360,586
Rv3001c
Rv3002c: Rv3002c - Probable acetolactate synthase (small subunit) IlvN (acetohydroxy-acid synthase) (AHAS) (ALS), at 3,360,624 to 3,361,130
Rv3002c
Rv3003c: Rv3003c - Acetolactate synthase (large subunit) IlvB1 (acetohydroxy-acid synthase), at 3,361,130 to 3,362,986
Rv3003c
Rv3004: Rv3004 - Low molecular weight protein antigen 6 (CFP-6), at 3,363,348 to 3,363,686
Rv3004
Rv3005c: Rv3005c - Conserved hypothetical protein, at 3,363,693 to 3,364,532
Rv3005c
Position (kb)
3361
3362
3363 Strain fitness (log2 ratio)
-2
-1
0
1 at 3360.622 kb on - strand at 3363.091 kb on - strand at 3363.118 kb on - strand at 3363.118 kb on - strand at 3363.153 kb on - strand at 3363.166 kb on - strand at 3363.398 kb on - strand, within Rv3004 at 3363.427 kb on + strand, within Rv3004 at 3363.766 kb on - strand at 3363.780 kb on - strand, within Rv3005c at 3363.780 kb on - strand, within Rv3005c at 3363.878 kb on - strand, within Rv3005c at 3363.878 kb on - strand, within Rv3005c at 3363.901 kb on + strand, within Rv3005c at 3363.901 kb on + strand, within Rv3005c at 3363.901 kb on + strand, within Rv3005c
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Hygromycin B 0.0095 mM remove 3,360,622 - -0.6 3,363,091 - +0.3 3,363,118 - -1.4 3,363,118 - +0.2 3,363,153 - -1.2 3,363,166 - +0.2 3,363,398 - Rv3004 0.15 +1.1 3,363,427 + Rv3004 0.23 -0.2 3,363,766 - +0.2 3,363,780 - Rv3005c 0.10 -0.3 3,363,780 - Rv3005c 0.10 +0.4 3,363,878 - Rv3005c 0.22 -2.1 3,363,878 - Rv3005c 0.22 -0.9 3,363,901 + Rv3005c 0.25 +0.2 3,363,901 + Rv3005c 0.25 -0.9 3,363,901 + Rv3005c 0.25 -0.6
Or see this region's nucleotide sequence