Experiment: 7H9 with Hygromycin B 0.0095 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv2528c and Rv2529 are separated by 203 nucleotides Rv2529 and Rv2530c are separated by 0 nucleotides Rv2530c and Rv2530A overlap by 4 nucleotides Rv2530A and Rv2531c are separated by 30 nucleotides
Rv2528c: Rv2528c - Probable restriction system protein Mrr, at 2,851,751 to 2,852,671
Rv2528c
Rv2529: Rv2529 - Hypothetical protein, at 2,852,875 to 2,854,266
Rv2529
Rv2530c: Rv2530c - Possible toxin VapC39. Contains PIN domain., at 2,854,267 to 2,854,686
Rv2530c
Rv2530A: Rv2530A - Possible antitoxin VapB39, at 2,854,683 to 2,854,907
Rv2530A
Rv2531c: Rv2531c - Probable amino acid decarboxylase, at 2,854,938 to 2,857,781
Rv2531c
Position (kb)
2852
2853
2854
2855 Strain fitness (log2 ratio)
-1
0
1 at 2852.017 kb on - strand, within Rv2528c at 2852.035 kb on - strand, within Rv2528c at 2852.506 kb on - strand, within Rv2528c at 2852.506 kb on - strand, within Rv2528c at 2852.625 kb on + strand at 2852.724 kb on + strand at 2852.814 kb on + strand at 2852.814 kb on + strand at 2852.828 kb on - strand at 2852.828 kb on - strand at 2852.828 kb on - strand at 2853.439 kb on + strand, within Rv2529 at 2853.439 kb on + strand, within Rv2529 at 2853.504 kb on - strand, within Rv2529 at 2853.935 kb on - strand, within Rv2529 at 2854.908 kb on - strand at 2855.040 kb on + strand at 2855.122 kb on + strand at 2855.122 kb on + strand at 2855.122 kb on + strand at 2855.122 kb on + strand at 2855.162 kb on - strand at 2855.162 kb on - strand at 2855.241 kb on + strand, within Rv2531c
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Hygromycin B 0.0095 mM remove 2,852,017 - Rv2528c 0.29 +0.2 2,852,035 - Rv2528c 0.31 -0.2 2,852,506 - Rv2528c 0.82 -1.2 2,852,506 - Rv2528c 0.82 -0.7 2,852,625 + -0.4 2,852,724 + +0.2 2,852,814 + -0.3 2,852,814 + +0.0 2,852,828 - -0.1 2,852,828 - +0.4 2,852,828 - -0.1 2,853,439 + Rv2529 0.41 -0.3 2,853,439 + Rv2529 0.41 -0.1 2,853,504 - Rv2529 0.45 -0.2 2,853,935 - Rv2529 0.76 -1.0 2,854,908 - -0.5 2,855,040 + -0.4 2,855,122 + -1.4 2,855,122 + -0.9 2,855,122 + -0.1 2,855,122 + -0.1 2,855,162 - -0.1 2,855,162 - +0.6 2,855,241 + Rv2531c 0.11 -0.2
Or see this region's nucleotide sequence