Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv1214c

Experiment: 7H9 with Hygromycin B 0.0095 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv1213 and Rv1214c are separated by 242 nucleotidesRv1214c and Rv1215c are separated by 133 nucleotides Rv1213: Rv1213 - Glucose-1-phosphate adenylyltransferase GlgC (ADP-glucose synthase) (ADP-glucose pyrophosphorylase), at 1,355,836 to 1,357,050 Rv1213 Rv1214c: Rv1214c - PE family protein PE14, at 1,357,293 to 1,357,625 Rv1214c Rv1215c: Rv1215c - Conserved protein, at 1,357,759 to 1,359,444 Rv1215c Position (kb) 1357 1358Strain fitness (log2 ratio) -1 0 1 2 3at 1356.294 kb on - strand, within Rv1213at 1356.330 kb on + strand, within Rv1213at 1356.330 kb on + strand, within Rv1213at 1356.437 kb on - strand, within Rv1213at 1356.559 kb on + strand, within Rv1213at 1356.610 kb on + strand, within Rv1213at 1356.654 kb on + strand, within Rv1213at 1356.654 kb on + strand, within Rv1213at 1356.654 kb on + strand, within Rv1213at 1356.655 kb on - strand, within Rv1213at 1356.691 kb on + strand, within Rv1213at 1356.691 kb on + strand, within Rv1213at 1356.692 kb on - strand, within Rv1213at 1357.049 kb on - strandat 1357.423 kb on - strand, within Rv1214cat 1357.423 kb on - strand, within Rv1214cat 1357.458 kb on - strand, within Rv1214cat 1357.469 kb on - strand, within Rv1214cat 1357.469 kb on - strand, within Rv1214cat 1357.645 kb on - strandat 1357.913 kb on - strandat 1358.105 kb on + strand, within Rv1215cat 1358.339 kb on + strand, within Rv1215cat 1358.507 kb on + strand, within Rv1215cat 1358.540 kb on + strand, within Rv1215cat 1358.540 kb on + strand, within Rv1215cat 1358.541 kb on - strand, within Rv1215c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Hygromycin B 0.0095 mM
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1,356,294 - Rv1213 0.38 -0.9
1,356,330 + Rv1213 0.41 -1.2
1,356,330 + Rv1213 0.41 +0.6
1,356,437 - Rv1213 0.49 +0.5
1,356,559 + Rv1213 0.60 +2.8
1,356,610 + Rv1213 0.64 -0.4
1,356,654 + Rv1213 0.67 -0.9
1,356,654 + Rv1213 0.67 -0.6
1,356,654 + Rv1213 0.67 -0.1
1,356,655 - Rv1213 0.67 -0.7
1,356,691 + Rv1213 0.70 -0.1
1,356,691 + Rv1213 0.70 +0.1
1,356,692 - Rv1213 0.70 +0.0
1,357,049 - +0.2
1,357,423 - Rv1214c 0.39 -0.6
1,357,423 - Rv1214c 0.39 +0.4
1,357,458 - Rv1214c 0.50 +1.4
1,357,469 - Rv1214c 0.53 +1.0
1,357,469 - Rv1214c 0.53 -0.1
1,357,645 - -0.0
1,357,913 - -0.1
1,358,105 + Rv1215c 0.21 -0.9
1,358,339 + Rv1215c 0.34 +0.1
1,358,507 + Rv1215c 0.44 +0.0
1,358,540 + Rv1215c 0.46 -1.4
1,358,540 + Rv1215c 0.46 +0.4
1,358,541 - Rv1215c 0.46 +0.4

Or see this region's nucleotide sequence