Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0210

Experiment: 7H9 with Hygromycin B 0.0095 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0209 and Rv0210 overlap by 4 nucleotidesRv0210 and Rv0211 are separated by 183 nucleotides Rv0209: Rv0209 - Hypothetical protein, at 249,038 to 250,123 Rv0209 Rv0210: Rv0210 - Hypothetical protein, at 250,120 to 251,598 Rv0210 Rv0211: Rv0211 - Probable iron-regulated phosphoenolpyruvate carboxykinase [GTP] PckA (phosphoenolpyruvate carboxylase) (PEPCK)(pep carboxykinase), at 251,782 to 253,602 Rv0211 Position (kb) 250 251 252Strain fitness (log2 ratio) -1 0 1 2at 249.282 kb on - strand, within Rv0209at 249.436 kb on + strand, within Rv0209at 249.437 kb on - strand, within Rv0209at 249.437 kb on - strand, within Rv0209at 249.619 kb on - strand, within Rv0209at 249.924 kb on + strand, within Rv0209at 249.925 kb on - strand, within Rv0209at 249.925 kb on - strand, within Rv0209at 250.004 kb on + strand, within Rv0209at 250.005 kb on - strand, within Rv0209at 250.303 kb on - strand, within Rv0210at 250.303 kb on - strand, within Rv0210at 250.440 kb on - strand, within Rv0210at 250.488 kb on + strand, within Rv0210at 250.488 kb on + strand, within Rv0210at 250.489 kb on - strand, within Rv0210at 250.489 kb on - strand, within Rv0210at 250.846 kb on + strand, within Rv0210at 251.400 kb on + strand, within Rv0210at 251.401 kb on - strand, within Rv0210at 251.690 kb on + strandat 251.730 kb on + strandat 251.734 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Hygromycin B 0.0095 mM
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249,282 - Rv0209 0.22 +0.1
249,436 + Rv0209 0.37 -0.6
249,437 - Rv0209 0.37 -0.4
249,437 - Rv0209 0.37 +0.8
249,619 - Rv0209 0.53 -1.3
249,924 + Rv0209 0.82 -0.3
249,925 - Rv0209 0.82 -0.3
249,925 - Rv0209 0.82 +1.6
250,004 + Rv0209 0.89 -1.7
250,005 - Rv0209 0.89 +1.1
250,303 - Rv0210 0.12 -0.2
250,303 - Rv0210 0.12 -0.1
250,440 - Rv0210 0.22 +0.7
250,488 + Rv0210 0.25 -0.6
250,488 + Rv0210 0.25 -0.8
250,489 - Rv0210 0.25 +0.3
250,489 - Rv0210 0.25 +2.1
250,846 + Rv0210 0.49 -0.9
251,400 + Rv0210 0.87 +0.6
251,401 - Rv0210 0.87 +0.0
251,690 + -0.5
251,730 + -0.3
251,734 + -1.4

Or see this region's nucleotide sequence