Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2476c

Experiment: 7H9 with Sulfaquinoxaline 0.097 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2474c and Rv2475c are separated by 5 nucleotidesRv2475c and Rv2476c overlap by 4 nucleotidesRv2476c and Rv2477c are separated by 103 nucleotides Rv2474c: Rv2474c - Conserved hypothetical protein, at 2,776,316 to 2,776,969 Rv2474c Rv2475c: Rv2475c - Conserved protein, at 2,776,975 to 2,777,391 Rv2475c Rv2476c: Rv2476c - Probable NAD-dependent glutamate dehydrogenase Gdh (NAD-Gdh) (NAD-dependent glutamic dehydrogenase), at 2,777,388 to 2,782,262 Rv2476c Rv2477c: Rv2477c - Probable macrolide-transport ATP-binding protein ABC transporter, at 2,782,366 to 2,784,042 Rv2477c Position (kb) 2777 2778 2779 2780 2781 2782 2783Strain fitness (log2 ratio) -1 0 1 2at 2776.641 kb on + strand, within Rv2474cat 2776.977 kb on - strandat 2777.000 kb on + strandat 2777.001 kb on - strandat 2777.211 kb on - strand, within Rv2475cat 2777.274 kb on + strand, within Rv2475cat 2777.336 kb on + strand, within Rv2475cat 2777.336 kb on + strand, within Rv2475cat 2777.336 kb on + strand, within Rv2475cat 2777.336 kb on + strand, within Rv2475cat 2777.611 kb on - strandat 2777.735 kb on - strandat 2777.830 kb on + strandat 2777.996 kb on - strand, within Rv2476cat 2778.137 kb on - strand, within Rv2476cat 2778.193 kb on + strand, within Rv2476cat 2778.515 kb on - strand, within Rv2476cat 2779.505 kb on + strand, within Rv2476cat 2779.506 kb on - strand, within Rv2476cat 2779.531 kb on + strand, within Rv2476cat 2779.531 kb on + strand, within Rv2476cat 2779.639 kb on + strand, within Rv2476cat 2779.959 kb on + strand, within Rv2476cat 2780.728 kb on + strand, within Rv2476cat 2781.043 kb on + strand, within Rv2476cat 2781.386 kb on - strand, within Rv2476cat 2781.404 kb on - strand, within Rv2476cat 2782.061 kb on - strandat 2782.160 kb on - strandat 2782.172 kb on - strandat 2782.361 kb on + strandat 2782.361 kb on + strandat 2782.362 kb on - strandat 2782.362 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Sulfaquinoxaline 0.097 mM
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2,776,641 + Rv2474c 0.50 -0.3
2,776,977 - +0.5
2,777,000 + +0.2
2,777,001 - -0.3
2,777,211 - Rv2475c 0.57 -0.5
2,777,274 + Rv2475c 0.72 +1.4
2,777,336 + Rv2475c 0.87 -0.2
2,777,336 + Rv2475c 0.87 +0.0
2,777,336 + Rv2475c 0.87 +0.4
2,777,336 + Rv2475c 0.87 -0.4
2,777,611 - -0.2
2,777,735 - -0.1
2,777,830 + -0.5
2,777,996 - Rv2476c 0.12 -0.2
2,778,137 - Rv2476c 0.15 +1.8
2,778,193 + Rv2476c 0.17 +0.4
2,778,515 - Rv2476c 0.23 -1.7
2,779,505 + Rv2476c 0.43 +0.7
2,779,506 - Rv2476c 0.43 +1.8
2,779,531 + Rv2476c 0.44 +0.6
2,779,531 + Rv2476c 0.44 +0.6
2,779,639 + Rv2476c 0.46 +2.3
2,779,959 + Rv2476c 0.53 -0.4
2,780,728 + Rv2476c 0.69 +0.8
2,781,043 + Rv2476c 0.75 -0.4
2,781,386 - Rv2476c 0.82 +0.8
2,781,404 - Rv2476c 0.82 +1.0
2,782,061 - +0.0
2,782,160 - +1.6
2,782,172 - +0.1
2,782,361 + -1.0
2,782,361 + +0.8
2,782,362 - -1.1
2,782,362 - -0.8

Or see this region's nucleotide sequence