Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2021c

Experiment: 7H9 with Sulfaquinoxaline 0.097 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2018 and Rv2019 overlap by 11 nucleotidesRv2019 and Rv2020c are separated by 15 nucleotidesRv2020c and Rv2021c are separated by 84 nucleotidesRv2021c and Rv2022c are separated by 8 nucleotidesRv2022c and Rv2023c are separated by 24 nucleotides Rv2018: Rv2018 - Conserved protein, at 2,265,280 to 2,265,999 Rv2018 Rv2019: Rv2019 - Conserved protein, at 2,265,989 to 2,266,405 Rv2019 Rv2020c: Rv2020c - Conserved hypothetical protein, at 2,266,421 to 2,266,720 Rv2020c Rv2021c: Rv2021c - Transcriptional regulatory protein, at 2,266,805 to 2,267,110 Rv2021c Rv2022c: Rv2022c - Conserved protein, at 2,267,119 to 2,267,724 Rv2022c Rv2023c: Rv2023c - Hypothetical protein, at 2,267,749 to 2,268,108 Rv2023c Position (kb) 2266 2267 2268Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2265.851 kb on - strand, within Rv2018at 2266.115 kb on + strand, within Rv2019at 2266.260 kb on + strand, within Rv2019at 2266.310 kb on + strand, within Rv2019at 2266.311 kb on - strand, within Rv2019at 2266.311 kb on - strand, within Rv2019at 2266.311 kb on - strand, within Rv2019at 2266.311 kb on - strand, within Rv2019at 2266.312 kb on + strand, within Rv2019at 2266.313 kb on - strand, within Rv2019at 2266.313 kb on - strand, within Rv2019at 2266.367 kb on + strandat 2266.367 kb on + strandat 2266.367 kb on + strandat 2266.368 kb on - strandat 2266.403 kb on + strandat 2266.403 kb on + strandat 2266.404 kb on - strandat 2266.404 kb on - strandat 2266.404 kb on - strandat 2266.500 kb on + strand, within Rv2020cat 2266.501 kb on - strand, within Rv2020cat 2266.501 kb on - strand, within Rv2020cat 2266.501 kb on - strand, within Rv2020cat 2266.622 kb on + strand, within Rv2020cat 2266.667 kb on + strand, within Rv2020cat 2266.668 kb on - strand, within Rv2020cat 2267.078 kb on + strand, within Rv2021cat 2267.079 kb on - strand, within Rv2021cat 2267.079 kb on - strand, within Rv2021cat 2267.096 kb on + strandat 2267.096 kb on + strandat 2267.096 kb on + strandat 2267.097 kb on - strandat 2267.097 kb on - strandat 2267.120 kb on + strandat 2267.121 kb on - strandat 2267.121 kb on - strandat 2267.121 kb on - strandat 2267.216 kb on + strand, within Rv2022cat 2267.244 kb on + strand, within Rv2022cat 2267.418 kb on + strand, within Rv2022cat 2267.438 kb on + strand, within Rv2022cat 2267.439 kb on - strand, within Rv2022cat 2267.515 kb on + strand, within Rv2022cat 2267.515 kb on + strand, within Rv2022cat 2267.516 kb on - strand, within Rv2022cat 2267.546 kb on + strand, within Rv2022cat 2267.558 kb on + strand, within Rv2022cat 2267.580 kb on + strand, within Rv2022cat 2267.641 kb on + strand, within Rv2022cat 2267.641 kb on + strand, within Rv2022cat 2267.641 kb on + strand, within Rv2022cat 2267.642 kb on - strand, within Rv2022cat 2267.674 kb on + strandat 2267.674 kb on + strandat 2267.675 kb on - strandat 2267.684 kb on + strandat 2267.684 kb on + strandat 2267.684 kb on + strandat 2267.871 kb on + strand, within Rv2023cat 2268.032 kb on + strand, within Rv2023cat 2268.032 kb on + strand, within Rv2023cat 2268.032 kb on + strand, within Rv2023c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Sulfaquinoxaline 0.097 mM
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2,265,851 - Rv2018 0.79 -0.3
2,266,115 + Rv2019 0.30 +0.5
2,266,260 + Rv2019 0.65 +0.1
2,266,310 + Rv2019 0.77 -0.4
2,266,311 - Rv2019 0.77 +0.2
2,266,311 - Rv2019 0.77 +0.0
2,266,311 - Rv2019 0.77 +0.2
2,266,311 - Rv2019 0.77 -0.7
2,266,312 + Rv2019 0.77 +0.1
2,266,313 - Rv2019 0.78 -0.4
2,266,313 - Rv2019 0.78 +0.3
2,266,367 + +0.8
2,266,367 + +0.9
2,266,367 + -1.2
2,266,368 - +1.8
2,266,403 + -0.1
2,266,403 + -2.3
2,266,404 - +0.0
2,266,404 - -0.4
2,266,404 - -2.0
2,266,500 + Rv2020c 0.26 -0.0
2,266,501 - Rv2020c 0.27 +0.1
2,266,501 - Rv2020c 0.27 -0.2
2,266,501 - Rv2020c 0.27 -0.1
2,266,622 + Rv2020c 0.67 +1.2
2,266,667 + Rv2020c 0.82 +0.4
2,266,668 - Rv2020c 0.82 -0.1
2,267,078 + Rv2021c 0.89 +1.0
2,267,079 - Rv2021c 0.90 -0.2
2,267,079 - Rv2021c 0.90 +0.4
2,267,096 + -0.5
2,267,096 + +0.5
2,267,096 + +0.4
2,267,097 - -0.4
2,267,097 - -0.3
2,267,120 + +0.1
2,267,121 - -0.7
2,267,121 - +0.0
2,267,121 - +0.8
2,267,216 + Rv2022c 0.16 -0.1
2,267,244 + Rv2022c 0.21 +0.8
2,267,418 + Rv2022c 0.49 +0.7
2,267,438 + Rv2022c 0.53 -0.2
2,267,439 - Rv2022c 0.53 -0.5
2,267,515 + Rv2022c 0.65 +0.6
2,267,515 + Rv2022c 0.65 +2.8
2,267,516 - Rv2022c 0.66 -0.1
2,267,546 + Rv2022c 0.70 +0.4
2,267,558 + Rv2022c 0.72 +0.0
2,267,580 + Rv2022c 0.76 +0.4
2,267,641 + Rv2022c 0.86 -0.0
2,267,641 + Rv2022c 0.86 -1.1
2,267,641 + Rv2022c 0.86 -0.5
2,267,642 - Rv2022c 0.86 +1.1
2,267,674 + -0.7
2,267,674 + +0.3
2,267,675 - -0.4
2,267,684 + -0.2
2,267,684 + -0.3
2,267,684 + -0.7
2,267,871 + Rv2023c 0.34 -0.9
2,268,032 + Rv2023c 0.79 -0.0
2,268,032 + Rv2023c 0.79 +0.1
2,268,032 + Rv2023c 0.79 -0.2

Or see this region's nucleotide sequence