Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0281

Experiment: 7H9 with Sulfamethizole 0.026 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0280 and Rv0281 are separated by 23 nucleotidesRv0281 and Rv0282 are separated by 223 nucleotides Rv0280: Rv0280 - PPE family protein PPE3, at 339,364 to 340,974 Rv0280 Rv0281: Rv0281 - Possible S-adenosylmethionine-dependent methyltransferase, at 340,998 to 341,906 Rv0281 Rv0282: Rv0282 - ESX conserved component EccA3. ESX-3 type VII secretion system protein., at 342,130 to 344,025 Rv0282 Position (kb) 340 341 342Strain fitness (log2 ratio) -1 0 1at 340.054 kb on + strand, within Rv0280at 340.182 kb on - strand, within Rv0280at 340.182 kb on - strand, within Rv0280at 340.182 kb on - strand, within Rv0280at 340.192 kb on + strand, within Rv0280at 340.339 kb on - strand, within Rv0280at 340.350 kb on - strand, within Rv0280at 340.525 kb on + strand, within Rv0280at 340.526 kb on - strand, within Rv0280at 340.531 kb on + strand, within Rv0280at 340.646 kb on + strand, within Rv0280at 340.646 kb on + strand, within Rv0280at 340.647 kb on - strand, within Rv0280at 340.697 kb on - strand, within Rv0280at 340.710 kb on + strand, within Rv0280at 340.710 kb on + strand, within Rv0280at 340.710 kb on + strand, within Rv0280at 340.710 kb on + strand, within Rv0280at 340.716 kb on + strand, within Rv0280at 340.717 kb on - strand, within Rv0280at 341.015 kb on + strandat 341.116 kb on - strand, within Rv0281at 341.247 kb on + strand, within Rv0281at 341.248 kb on - strand, within Rv0281at 341.248 kb on - strand, within Rv0281at 341.260 kb on - strand, within Rv0281at 341.260 kb on - strand, within Rv0281at 341.550 kb on + strand, within Rv0281at 341.666 kb on + strand, within Rv0281at 341.734 kb on - strand, within Rv0281at 341.812 kb on - strand, within Rv0281at 341.968 kb on + strandat 341.969 kb on - strandat 341.988 kb on - strandat 342.004 kb on - strandat 342.032 kb on - strandat 342.032 kb on - strandat 342.140 kb on + strandat 342.215 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Sulfamethizole 0.026 mM
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340,054 + Rv0280 0.43 +0.8
340,182 - Rv0280 0.51 -0.1
340,182 - Rv0280 0.51 -0.6
340,182 - Rv0280 0.51 +0.1
340,192 + Rv0280 0.51 -1.1
340,339 - Rv0280 0.61 -0.3
340,350 - Rv0280 0.61 -0.1
340,525 + Rv0280 0.72 -0.0
340,526 - Rv0280 0.72 -0.3
340,531 + Rv0280 0.72 -0.3
340,646 + Rv0280 0.80 -0.0
340,646 + Rv0280 0.80 +0.3
340,647 - Rv0280 0.80 +0.1
340,697 - Rv0280 0.83 +0.2
340,710 + Rv0280 0.84 -0.4
340,710 + Rv0280 0.84 -1.7
340,710 + Rv0280 0.84 -0.1
340,710 + Rv0280 0.84 -0.3
340,716 + Rv0280 0.84 -0.5
340,717 - Rv0280 0.84 -0.2
341,015 + -1.3
341,116 - Rv0281 0.13 +0.3
341,247 + Rv0281 0.27 -0.8
341,248 - Rv0281 0.28 +0.0
341,248 - Rv0281 0.28 -1.1
341,260 - Rv0281 0.29 -0.1
341,260 - Rv0281 0.29 -0.5
341,550 + Rv0281 0.61 -0.4
341,666 + Rv0281 0.73 -0.1
341,734 - Rv0281 0.81 -0.0
341,812 - Rv0281 0.90 -0.7
341,968 + -1.1
341,969 - +0.2
341,988 - -1.1
342,004 - -0.6
342,032 - -0.0
342,032 - -0.6
342,140 + -0.4
342,215 + -0.1

Or see this region's nucleotide sequence