Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3262

Experiment: 7H9 with Pretomanid 0.213 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3261 and Rv3262 overlap by 4 nucleotidesRv3262 and Rv3263 are separated by 295 nucleotides Rv3261: Rv3261 - Probable F420 biosynthesis protein FbiA, at 3,640,543 to 3,641,538 Rv3261 Rv3262: Rv3262 - Probable F420 biosynthesis protein FbiB, at 3,641,535 to 3,642,881 Rv3262 Rv3263: Rv3263 - Probable DNA methylase (modification methylase) (methyltransferase), at 3,643,177 to 3,644,838 Rv3263 Position (kb) 3641 3642 3643Strain fitness (log2 ratio) -1 0 1 2 3 4 5at 3640.741 kb on + strand, within Rv3261at 3640.742 kb on - strand, within Rv3261at 3640.742 kb on - strand, within Rv3261at 3640.742 kb on - strand, within Rv3261at 3641.003 kb on + strand, within Rv3261at 3641.887 kb on + strand, within Rv3262at 3642.060 kb on + strand, within Rv3262at 3642.061 kb on - strand, within Rv3262at 3642.526 kb on + strand, within Rv3262at 3643.037 kb on + strandat 3643.114 kb on + strandat 3643.114 kb on + strandat 3643.115 kb on - strandat 3643.219 kb on + strandat 3643.220 kb on - strandat 3643.240 kb on - strandat 3643.240 kb on - strandat 3643.240 kb on - strandat 3643.304 kb on - strandat 3643.306 kb on + strandat 3643.306 kb on + strandat 3643.306 kb on + strandat 3643.307 kb on - strandat 3643.507 kb on - strand, within Rv3263at 3643.634 kb on + strand, within Rv3263at 3643.764 kb on - strand, within Rv3263at 3643.764 kb on - strand, within Rv3263at 3643.807 kb on - strand, within Rv3263

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Pretomanid 0.213 ug/mL
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3,640,741 + Rv3261 0.20 +3.9
3,640,742 - Rv3261 0.20 +4.7
3,640,742 - Rv3261 0.20 +2.1
3,640,742 - Rv3261 0.20 +4.5
3,641,003 + Rv3261 0.46 +5.4
3,641,887 + Rv3262 0.26 +4.5
3,642,060 + Rv3262 0.39 +3.0
3,642,061 - Rv3262 0.39 +2.6
3,642,526 + Rv3262 0.74 +3.2
3,643,037 + +0.5
3,643,114 + +0.2
3,643,114 + -1.7
3,643,115 - -0.3
3,643,219 + +0.5
3,643,220 - +0.9
3,643,240 - -0.4
3,643,240 - +1.1
3,643,240 - +0.2
3,643,304 - -0.4
3,643,306 + +0.4
3,643,306 + -0.1
3,643,306 + -0.8
3,643,307 - +0.6
3,643,507 - Rv3263 0.20 +0.1
3,643,634 + Rv3263 0.27 +0.1
3,643,764 - Rv3263 0.35 +0.4
3,643,764 - Rv3263 0.35 +1.2
3,643,807 - Rv3263 0.38 -0.2

Or see this region's nucleotide sequence