Experiment: 7H9 with Pretomanid 0.213 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0404 and Rv0405 overlap by 4 nucleotides Rv0405 and Rv0406c overlap by 53 nucleotides Rv0406c and Rv0407 are separated by 77 nucleotides
Rv0404: Rv0404 - Fatty-acid-AMP ligase FadD30 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase), at 483,977 to 485,734
Rv0404
Rv0405: Rv0405 - Probable membrane bound polyketide synthase Pks6, at 485,731 to 489,939
Rv0405
Rv0406c: Rv0406c - Beta lactamase like protein, at 489,887 to 490,705
Rv0406c
Rv0407: Rv0407 - F420-dependent glucose-6-phosphate dehydrogenase Fgd1, at 490,783 to 491,793
Rv0407
Position (kb) 485
486
487
488
489
490 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 485.041 kb on + strand, within Rv0404 at 485.341 kb on - strand, within Rv0404 at 485.384 kb on - strand, within Rv0404 at 486.044 kb on + strand at 486.045 kb on - strand at 486.560 kb on + strand, within Rv0405 at 486.561 kb on - strand, within Rv0405 at 486.561 kb on - strand, within Rv0405 at 486.984 kb on - strand, within Rv0405 at 487.050 kb on + strand, within Rv0405 at 487.193 kb on + strand, within Rv0405 at 487.194 kb on - strand, within Rv0405 at 487.194 kb on - strand, within Rv0405 at 487.194 kb on - strand, within Rv0405 at 487.429 kb on + strand, within Rv0405 at 487.429 kb on + strand, within Rv0405 at 487.430 kb on - strand, within Rv0405 at 488.282 kb on - strand, within Rv0405 at 488.329 kb on + strand, within Rv0405 at 488.330 kb on - strand, within Rv0405 at 488.379 kb on + strand, within Rv0405 at 488.380 kb on - strand, within Rv0405 at 488.380 kb on - strand, within Rv0405 at 488.380 kb on - strand, within Rv0405 at 488.380 kb on - strand, within Rv0405 at 488.621 kb on - strand, within Rv0405 at 488.621 kb on - strand, within Rv0405 at 488.798 kb on + strand, within Rv0405 at 488.799 kb on - strand, within Rv0405 at 488.799 kb on - strand, within Rv0405 at 488.799 kb on - strand, within Rv0405 at 488.799 kb on - strand, within Rv0405 at 488.903 kb on + strand, within Rv0405 at 488.903 kb on + strand, within Rv0405 at 488.903 kb on + strand, within Rv0405 at 488.903 kb on + strand, within Rv0405 at 488.904 kb on - strand, within Rv0405 at 488.908 kb on - strand, within Rv0405 at 488.915 kb on + strand, within Rv0405 at 488.916 kb on - strand, within Rv0405 at 488.933 kb on + strand, within Rv0405 at 488.934 kb on - strand, within Rv0405 at 488.989 kb on + strand, within Rv0405 at 489.005 kb on + strand, within Rv0405 at 489.532 kb on - strand at 489.535 kb on + strand at 489.535 kb on + strand at 489.536 kb on - strand at 489.680 kb on - strand at 489.937 kb on + strand at 490.064 kb on + strand, within Rv0406c at 490.064 kb on + strand, within Rv0406c at 490.065 kb on - strand, within Rv0406c at 490.143 kb on + strand, within Rv0406c at 490.144 kb on - strand, within Rv0406c at 490.144 kb on - strand, within Rv0406c at 490.282 kb on + strand, within Rv0406c at 490.370 kb on + strand, within Rv0406c at 490.390 kb on - strand, within Rv0406c at 490.488 kb on + strand, within Rv0406c at 490.488 kb on + strand, within Rv0406c at 490.758 kb on + strand at 490.759 kb on - strand at 490.765 kb on + strand at 490.766 kb on - strand at 490.766 kb on - strand at 490.771 kb on + strand at 490.771 kb on + strand at 490.772 kb on - strand at 490.772 kb on - strand at 490.772 kb on - strand at 490.799 kb on + strand at 490.804 kb on + strand at 490.804 kb on + strand at 490.805 kb on - strand at 490.805 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Pretomanid 0.213 ug/mL remove 485,041 + Rv0404 0.61 +0.0 485,341 - Rv0404 0.78 -0.1 485,384 - Rv0404 0.80 -1.4 486,044 + +0.7 486,045 - -0.6 486,560 + Rv0405 0.20 +0.2 486,561 - Rv0405 0.20 -0.2 486,561 - Rv0405 0.20 -0.3 486,984 - Rv0405 0.30 -1.4 487,050 + Rv0405 0.31 -0.4 487,193 + Rv0405 0.35 +3.0 487,194 - Rv0405 0.35 +0.6 487,194 - Rv0405 0.35 +0.6 487,194 - Rv0405 0.35 -0.8 487,429 + Rv0405 0.40 +1.1 487,429 + Rv0405 0.40 -0.0 487,430 - Rv0405 0.40 -0.4 488,282 - Rv0405 0.61 +1.8 488,329 + Rv0405 0.62 +0.5 488,330 - Rv0405 0.62 +2.7 488,379 + Rv0405 0.63 +0.3 488,380 - Rv0405 0.63 -1.1 488,380 - Rv0405 0.63 -0.4 488,380 - Rv0405 0.63 +0.3 488,380 - Rv0405 0.63 -0.3 488,621 - Rv0405 0.69 -0.5 488,621 - Rv0405 0.69 +0.0 488,798 + Rv0405 0.73 +0.0 488,799 - Rv0405 0.73 -0.3 488,799 - Rv0405 0.73 +0.1 488,799 - Rv0405 0.73 -0.2 488,799 - Rv0405 0.73 +0.0 488,903 + Rv0405 0.75 -1.6 488,903 + Rv0405 0.75 -0.3 488,903 + Rv0405 0.75 +0.0 488,903 + Rv0405 0.75 +0.2 488,904 - Rv0405 0.75 +0.5 488,908 - Rv0405 0.75 +0.2 488,915 + Rv0405 0.76 -0.2 488,916 - Rv0405 0.76 +1.1 488,933 + Rv0405 0.76 +0.4 488,934 - Rv0405 0.76 -0.6 488,989 + Rv0405 0.77 +0.3 489,005 + Rv0405 0.78 -0.2 489,532 - -1.1 489,535 + +1.8 489,535 + -0.2 489,536 - -0.2 489,680 - +0.4 489,937 + -0.4 490,064 + Rv0406c 0.22 +2.6 490,064 + Rv0406c 0.22 -0.8 490,065 - Rv0406c 0.22 -0.6 490,143 + Rv0406c 0.31 +0.1 490,144 - Rv0406c 0.31 -0.5 490,144 - Rv0406c 0.31 -0.9 490,282 + Rv0406c 0.48 -2.5 490,370 + Rv0406c 0.59 -0.8 490,390 - Rv0406c 0.61 -1.8 490,488 + Rv0406c 0.73 -1.7 490,488 + Rv0406c 0.73 +0.2 490,758 + +0.1 490,759 - +0.2 490,765 + +0.7 490,766 - +0.5 490,766 - +0.7 490,771 + +2.4 490,771 + +1.2 490,772 - +0.1 490,772 - +0.7 490,772 - +0.2 490,799 + +0.9 490,804 + +4.0 490,804 + +0.7 490,805 - +4.3 490,805 - +4.5
Or see this region's nucleotide sequence