Experiment: 7H9 with Pretomanid 0.213 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0250c and Rv0251c are separated by 144 nucleotides Rv0251c and Rv0252 are separated by 213 nucleotides Rv0252 and Rv0253 are separated by 25 nucleotides Rv0253 and Rv0254c are separated by 15 nucleotides Rv0254c and Rv0255c are separated by 24 nucleotides
Rv0250c: Rv0250c - Conserved protein, at 301,735 to 302,028
Rv0250c
Rv0251c: Rv0251c - Heat shock protein Hsp (heat-stress-induced ribosome-binding protein A), at 302,173 to 302,652
Rv0251c
Rv0252: Rv0252 - Probable nitrite reductase [NAD(P)H] large subunit [FAD flavoprotein] NirB, at 302,866 to 305,427
Rv0252
Rv0253: Rv0253 - Probable nitrite reductase [NAD(P)H] small subunit NirD, at 305,453 to 305,809
Rv0253
Rv0254c: Rv0254c - Probable bifunctional cobalamin biosynthesis protein CobU: cobinamide kinase + cobinamide phosphate guanylyltransferase, at 305,825 to 306,349
Rv0254c
Rv0255c: Rv0255c - Probable cobyric acid synthase CobQ1, at 306,374 to 307,858
Rv0255c
Position (kb) 302
303
304
305
306 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 302.054 kb on + strand at 302.066 kb on + strand at 302.080 kb on + strand at 302.080 kb on + strand at 302.080 kb on + strand at 302.080 kb on + strand at 302.081 kb on - strand at 302.081 kb on - strand at 302.081 kb on - strand at 302.081 kb on - strand at 302.085 kb on - strand at 302.085 kb on - strand at 302.085 kb on - strand at 302.174 kb on + strand at 302.174 kb on + strand at 302.175 kb on - strand at 302.223 kb on - strand, within Rv0251c at 302.261 kb on + strand, within Rv0251c at 302.261 kb on + strand, within Rv0251c at 302.261 kb on + strand, within Rv0251c at 302.710 kb on + strand at 302.710 kb on + strand at 302.710 kb on + strand at 302.712 kb on + strand at 302.713 kb on - strand at 302.811 kb on + strand at 302.812 kb on - strand at 302.812 kb on - strand at 302.812 kb on - strand at 302.812 kb on - strand at 302.854 kb on + strand at 302.855 kb on - strand at 302.871 kb on + strand at 302.872 kb on - strand at 302.872 kb on - strand at 303.025 kb on + strand at 303.025 kb on + strand at 303.026 kb on - strand at 303.049 kb on + strand at 303.049 kb on + strand at 303.049 kb on + strand at 303.049 kb on + strand at 303.049 kb on + strand at 303.050 kb on - strand at 303.050 kb on - strand at 303.126 kb on - strand, within Rv0252 at 303.200 kb on - strand, within Rv0252 at 303.229 kb on + strand, within Rv0252 at 303.230 kb on - strand, within Rv0252 at 303.281 kb on - strand, within Rv0252 at 303.752 kb on - strand, within Rv0252 at 303.812 kb on - strand, within Rv0252 at 303.988 kb on + strand, within Rv0252 at 303.989 kb on - strand, within Rv0252 at 303.989 kb on - strand, within Rv0252 at 303.989 kb on - strand, within Rv0252 at 303.989 kb on - strand, within Rv0252 at 303.989 kb on - strand, within Rv0252 at 304.061 kb on - strand, within Rv0252 at 304.155 kb on - strand, within Rv0252 at 304.325 kb on + strand, within Rv0252 at 304.326 kb on - strand, within Rv0252 at 304.597 kb on + strand, within Rv0252 at 304.684 kb on + strand, within Rv0252 at 304.685 kb on - strand, within Rv0252 at 304.796 kb on - strand, within Rv0252 at 304.908 kb on - strand, within Rv0252 at 304.925 kb on + strand, within Rv0252 at 304.925 kb on + strand, within Rv0252 at 304.925 kb on + strand, within Rv0252 at 304.926 kb on - strand, within Rv0252 at 305.018 kb on - strand, within Rv0252 at 305.018 kb on - strand, within Rv0252 at 305.060 kb on + strand, within Rv0252 at 305.119 kb on + strand, within Rv0252 at 305.123 kb on - strand, within Rv0252 at 305.266 kb on + strand at 305.267 kb on - strand at 305.406 kb on + strand at 305.406 kb on + strand at 305.479 kb on - strand at 305.753 kb on + strand, within Rv0253 at 305.808 kb on - strand at 305.808 kb on - strand at 305.861 kb on + strand at 305.861 kb on + strand at 306.039 kb on + strand, within Rv0254c
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Pretomanid 0.213 ug/mL remove 302,054 + +0.2 302,066 + +0.3 302,080 + +0.1 302,080 + -0.1 302,080 + +1.9 302,080 + -0.0 302,081 - -0.2 302,081 - +1.0 302,081 - +1.9 302,081 - -2.5 302,085 - -0.5 302,085 - -0.3 302,085 - +0.1 302,174 + -0.4 302,174 + +1.2 302,175 - -1.1 302,223 - Rv0251c 0.10 +0.6 302,261 + Rv0251c 0.18 +0.6 302,261 + Rv0251c 0.18 -0.6 302,261 + Rv0251c 0.18 +0.0 302,710 + -0.1 302,710 + +0.3 302,710 + +0.3 302,712 + -0.9 302,713 - +0.7 302,811 + -0.6 302,812 - +0.1 302,812 - +0.7 302,812 - +3.4 302,812 - -0.1 302,854 + -0.0 302,855 - -0.6 302,871 + -0.5 302,872 - +0.0 302,872 - -0.4 303,025 + +0.1 303,025 + -0.4 303,026 - +0.3 303,049 + +1.1 303,049 + -0.4 303,049 + -0.5 303,049 + -0.1 303,049 + +1.9 303,050 - -0.0 303,050 - -0.6 303,126 - Rv0252 0.10 +0.3 303,200 - Rv0252 0.13 -0.9 303,229 + Rv0252 0.14 -0.4 303,230 - Rv0252 0.14 -0.3 303,281 - Rv0252 0.16 -1.6 303,752 - Rv0252 0.35 -0.2 303,812 - Rv0252 0.37 +0.4 303,988 + Rv0252 0.44 +0.0 303,989 - Rv0252 0.44 -0.3 303,989 - Rv0252 0.44 -0.2 303,989 - Rv0252 0.44 +0.6 303,989 - Rv0252 0.44 +0.3 303,989 - Rv0252 0.44 -0.7 304,061 - Rv0252 0.47 +0.6 304,155 - Rv0252 0.50 +0.7 304,325 + Rv0252 0.57 +0.3 304,326 - Rv0252 0.57 -0.3 304,597 + Rv0252 0.68 +0.1 304,684 + Rv0252 0.71 +0.2 304,685 - Rv0252 0.71 -0.0 304,796 - Rv0252 0.75 +0.9 304,908 - Rv0252 0.80 -0.7 304,925 + Rv0252 0.80 -1.1 304,925 + Rv0252 0.80 +0.6 304,925 + Rv0252 0.80 -0.5 304,926 - Rv0252 0.80 +0.7 305,018 - Rv0252 0.84 +0.3 305,018 - Rv0252 0.84 +1.3 305,060 + Rv0252 0.86 +0.1 305,119 + Rv0252 0.88 +2.6 305,123 - Rv0252 0.88 +0.2 305,266 + -0.0 305,267 - -0.3 305,406 + -0.5 305,406 + +0.1 305,479 - -0.1 305,753 + Rv0253 0.84 -0.5 305,808 - -1.7 305,808 - +1.6 305,861 + +0.1 305,861 + -0.5 306,039 + Rv0254c 0.41 +2.1
Or see this region's nucleotide sequence