Experiment: 7H9 with Meropenem 9.6 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv1821 and Rv1822 are separated by 196 nucleotides Rv1822 and Rv1823 overlap by 8 nucleotides Rv1823 and Rv1824 are separated by 28 nucleotides
Rv1821: Rv1821 - Possible preprotein translocase ATPase SecA2, at 2,066,457 to 2,068,883
Rv1821
Rv1822: Rv1822 - Probable CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase PgsA2 (PGP synthase) (phosphatidylglycerophosphate synthase) (3-phosphatidyl-1'-glycerol-3'phosphate synthase), at 2,069,080 to 2,069,709
Rv1822
Rv1823: Rv1823 - Conserved protein, at 2,069,702 to 2,070,625
Rv1823
Rv1824: Rv1824 - Conserved hypothetical membrane protein, at 2,070,654 to 2,071,019
Rv1824
Position (kb)
2069
2070 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2068.457 kb on - strand, within Rv1821 at 2068.813 kb on - strand at 2068.813 kb on - strand at 2068.813 kb on - strand at 2068.813 kb on - strand at 2068.896 kb on - strand at 2068.909 kb on - strand at 2069.010 kb on + strand at 2069.010 kb on + strand at 2069.814 kb on + strand, within Rv1823 at 2070.160 kb on + strand, within Rv1823 at 2070.161 kb on - strand, within Rv1823 at 2070.430 kb on + strand, within Rv1823 at 2070.438 kb on - strand, within Rv1823 at 2070.530 kb on + strand, within Rv1823 at 2070.531 kb on - strand, within Rv1823 at 2070.623 kb on + strand at 2070.645 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Meropenem 9.6 ug/mL remove 2,068,457 - Rv1821 0.82 -0.8 2,068,813 - -1.4 2,068,813 - -0.2 2,068,813 - -1.7 2,068,813 - +0.8 2,068,896 - +4.1 2,068,909 - -0.1 2,069,010 + +0.2 2,069,010 + -0.0 2,069,814 + Rv1823 0.12 -0.2 2,070,160 + Rv1823 0.50 -0.3 2,070,161 - Rv1823 0.50 -0.3 2,070,430 + Rv1823 0.79 -0.6 2,070,438 - Rv1823 0.80 +0.0 2,070,530 + Rv1823 0.90 -0.5 2,070,531 - Rv1823 0.90 -1.0 2,070,623 + +0.0 2,070,645 + -0.1
Or see this region's nucleotide sequence