Experiment: 7H9 with Dimethyl Sulfoxide 1 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3144c and Rv3145 are separated by 364 nucleotides Rv3145 and Rv3146 are separated by 8 nucleotides Rv3146 and Rv3147 overlap by 4 nucleotides Rv3147 and Rv3148 overlap by 1 nucleotides
Rv3144c: Rv3144c - PPE family protein PPE52, at 3,510,088 to 3,511,317
Rv3144c
Rv3145: Rv3145 - Probable NADH dehydrogenase I (chain A) NuoA (NADH-ubiquinone oxidoreductase chain A), at 3,511,682 to 3,512,068
Rv3145
Rv3146: Rv3146 - Probable NADH dehydrogenase I (chain B) NuoB (NADH-ubiquinone oxidoreductase chain B), at 3,512,077 to 3,512,631
Rv3146
Rv3147: Rv3147 - Probable NADH dehydrogenase I (chain C) NuoC (NADH-ubiquinone oxidoreductase chain C), at 3,512,628 to 3,513,338
Rv3147
Rv3148: Rv3148 - Probable NADH dehydrogenase I (chain D) NuoD (NADH-ubiquinone oxidoreductase chain D), at 3,513,338 to 3,514,660
Rv3148
Position (kb)
3512
3513 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3511.104 kb on - strand, within Rv3144c at 3511.416 kb on + strand at 3511.416 kb on + strand at 3511.427 kb on + strand at 3511.441 kb on + strand at 3511.441 kb on + strand at 3511.441 kb on + strand at 3511.530 kb on + strand at 3511.530 kb on + strand at 3511.530 kb on + strand at 3511.530 kb on + strand at 3511.530 kb on + strand at 3511.530 kb on + strand at 3511.531 kb on - strand at 3511.531 kb on - strand at 3511.531 kb on - strand at 3511.531 kb on - strand at 3511.576 kb on + strand at 3511.691 kb on + strand at 3511.691 kb on + strand at 3511.691 kb on + strand at 3511.692 kb on - strand at 3511.692 kb on - strand at 3511.695 kb on + strand at 3511.707 kb on + strand at 3511.825 kb on + strand, within Rv3145 at 3511.889 kb on + strand, within Rv3145 at 3511.889 kb on + strand, within Rv3145 at 3511.890 kb on - strand, within Rv3145 at 3511.890 kb on - strand, within Rv3145 at 3511.890 kb on - strand, within Rv3145 at 3511.893 kb on - strand, within Rv3145 at 3511.893 kb on - strand, within Rv3145 at 3511.893 kb on - strand, within Rv3145 at 3512.002 kb on - strand, within Rv3145 at 3512.033 kb on + strand at 3512.033 kb on + strand at 3512.034 kb on - strand at 3512.034 kb on - strand at 3512.034 kb on - strand at 3512.140 kb on + strand, within Rv3146 at 3512.338 kb on + strand, within Rv3146 at 3512.338 kb on + strand, within Rv3146 at 3512.459 kb on - strand, within Rv3146 at 3512.540 kb on + strand, within Rv3146 at 3512.540 kb on + strand, within Rv3146 at 3512.544 kb on + strand, within Rv3146 at 3512.927 kb on - strand, within Rv3147 at 3513.007 kb on - strand, within Rv3147 at 3513.263 kb on - strand, within Rv3147 at 3513.484 kb on + strand, within Rv3148 at 3513.484 kb on + strand, within Rv3148
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Dimethyl Sulfoxide 1 vol% remove 3,511,104 - Rv3144c 0.83 +0.3 3,511,416 + -0.1 3,511,416 + -0.3 3,511,427 + -0.1 3,511,441 + +0.6 3,511,441 + -0.3 3,511,441 + -1.4 3,511,530 + -0.3 3,511,530 + -0.1 3,511,530 + +0.0 3,511,530 + -0.4 3,511,530 + -0.4 3,511,530 + -0.2 3,511,531 - +0.3 3,511,531 - -0.1 3,511,531 - +0.2 3,511,531 - -0.4 3,511,576 + -0.4 3,511,691 + -0.3 3,511,691 + +0.8 3,511,691 + -0.1 3,511,692 - -0.1 3,511,692 - -0.4 3,511,695 + -0.3 3,511,707 + -0.6 3,511,825 + Rv3145 0.37 +0.5 3,511,889 + Rv3145 0.53 +2.1 3,511,889 + Rv3145 0.53 -0.6 3,511,890 - Rv3145 0.54 -0.1 3,511,890 - Rv3145 0.54 -0.6 3,511,890 - Rv3145 0.54 -0.3 3,511,893 - Rv3145 0.55 -0.5 3,511,893 - Rv3145 0.55 +0.1 3,511,893 - Rv3145 0.55 +0.2 3,512,002 - Rv3145 0.83 -0.7 3,512,033 + -1.2 3,512,033 + +2.1 3,512,034 - +0.8 3,512,034 - -1.8 3,512,034 - -1.5 3,512,140 + Rv3146 0.11 -0.6 3,512,338 + Rv3146 0.47 -0.4 3,512,338 + Rv3146 0.47 -0.8 3,512,459 - Rv3146 0.69 -0.2 3,512,540 + Rv3146 0.83 -0.3 3,512,540 + Rv3146 0.83 -1.4 3,512,544 + Rv3146 0.84 -1.3 3,512,927 - Rv3147 0.42 -0.4 3,513,007 - Rv3147 0.53 -0.8 3,513,263 - Rv3147 0.89 +0.2 3,513,484 + Rv3148 0.11 -0.8 3,513,484 + Rv3148 0.11 +0.5
Or see this region's nucleotide sequence