Experiment: 7H9 with Dimethyl Sulfoxide 1 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv2622 and Rv2623 are separated by 135 nucleotides Rv2623 and Rv2624c are separated by 2 nucleotides Rv2624c and Rv2625c are separated by 14 nucleotides
Rv2622: Rv2622 - Possible methyltransferase (methylase), at 2,948,636 to 2,949,457
Rv2622
Rv2623: Rv2623 - Universal stress protein family protein TB31.7, at 2,949,593 to 2,950,486
Rv2623
Rv2624c: Rv2624c - Universal stress protein family protein, at 2,950,489 to 2,951,307
Rv2624c
Rv2625c: Rv2625c - Probable conserved transmembrane alanine and leucine rich protein, at 2,951,322 to 2,952,503
Rv2625c
Position (kb)
2949
2950
2951 Strain fitness (log2 ratio)
-1
0
1 at 2948.635 kb on + strand at 2948.635 kb on + strand at 2948.635 kb on + strand at 2948.635 kb on + strand at 2948.635 kb on + strand at 2948.636 kb on - strand at 2948.873 kb on + strand, within Rv2622 at 2948.873 kb on + strand, within Rv2622 at 2949.277 kb on - strand, within Rv2622 at 2949.485 kb on + strand at 2949.623 kb on - strand at 2949.735 kb on + strand, within Rv2623 at 2950.080 kb on + strand, within Rv2623 at 2950.081 kb on - strand, within Rv2623 at 2950.081 kb on - strand, within Rv2623 at 2950.485 kb on - strand at 2950.661 kb on + strand, within Rv2624c at 2951.141 kb on + strand, within Rv2624c at 2951.263 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Dimethyl Sulfoxide 1 vol% remove 2,948,635 + +0.1 2,948,635 + +0.8 2,948,635 + -0.4 2,948,635 + +0.5 2,948,635 + -0.2 2,948,636 - -0.3 2,948,873 + Rv2622 0.29 +1.0 2,948,873 + Rv2622 0.29 -0.8 2,949,277 - Rv2622 0.78 -0.3 2,949,485 + +0.9 2,949,623 - -0.3 2,949,735 + Rv2623 0.16 +0.9 2,950,080 + Rv2623 0.54 +0.3 2,950,081 - Rv2623 0.55 +1.1 2,950,081 - Rv2623 0.55 +0.2 2,950,485 - -0.6 2,950,661 + Rv2624c 0.21 +0.8 2,951,141 + Rv2624c 0.80 -1.0 2,951,263 - -1.5
Or see this region's nucleotide sequence