Experiment: 7H9 with Dimethyl Sulfoxide 1 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv2008c and Rv2009 are separated by 87 nucleotides Rv2009 and Rv2010 are separated by 0 nucleotides Rv2010 and Rv2011c are separated by 182 nucleotides Rv2011c and Rv2012 are separated by 40 nucleotides
Rv2008c: Rv2008c - Conserved hypothetical protein, at 2,256,617 to 2,257,942
Rv2008c
Rv2009: Rv2009 - Antitoxin VapB15, at 2,258,030 to 2,258,272
Rv2009
Rv2010: Rv2010 - Toxin VapC15, at 2,258,273 to 2,258,671
Rv2010
Rv2011c: Rv2011c - Conserved hypothetical protein, probable transcription repressor., at 2,258,854 to 2,259,285
Rv2011c
Rv2012: Rv2012 - Conserved hypothetical protein, at 2,259,326 to 2,259,820
Rv2012
Position (kb)
2258
2259
2260 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2257.865 kb on + strand at 2257.866 kb on - strand at 2258.008 kb on + strand at 2258.008 kb on + strand at 2258.008 kb on + strand at 2258.008 kb on + strand at 2258.008 kb on + strand at 2258.009 kb on - strand at 2258.102 kb on + strand, within Rv2009 at 2258.103 kb on - strand, within Rv2009 at 2258.270 kb on + strand at 2258.271 kb on - strand at 2258.271 kb on - strand at 2258.303 kb on + strand at 2258.544 kb on + strand, within Rv2010 at 2258.545 kb on - strand, within Rv2010 at 2258.545 kb on - strand, within Rv2010 at 2258.738 kb on - strand at 2258.747 kb on + strand at 2258.790 kb on - strand at 2259.229 kb on + strand, within Rv2011c at 2259.230 kb on - strand, within Rv2011c at 2259.230 kb on - strand, within Rv2011c at 2259.250 kb on + strand at 2259.537 kb on - strand, within Rv2012 at 2259.548 kb on + strand, within Rv2012 at 2259.548 kb on + strand, within Rv2012 at 2259.549 kb on - strand, within Rv2012 at 2259.549 kb on - strand, within Rv2012 at 2259.710 kb on + strand, within Rv2012 at 2259.744 kb on - strand, within Rv2012 at 2259.744 kb on - strand, within Rv2012 at 2259.767 kb on + strand, within Rv2012 at 2259.767 kb on + strand, within Rv2012 at 2259.768 kb on - strand, within Rv2012 at 2259.768 kb on - strand, within Rv2012 at 2259.770 kb on - strand, within Rv2012 at 2260.023 kb on - strand at 2260.080 kb on + strand at 2260.081 kb on - strand at 2260.081 kb on - strand at 2260.171 kb on + strand at 2260.172 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Dimethyl Sulfoxide 1 vol% remove 2,257,865 + -2.0 2,257,866 - -0.5 2,258,008 + +0.0 2,258,008 + -0.2 2,258,008 + -0.3 2,258,008 + +1.0 2,258,008 + +0.5 2,258,009 - -0.2 2,258,102 + Rv2009 0.30 +1.2 2,258,103 - Rv2009 0.30 -0.0 2,258,270 + -0.2 2,258,271 - +0.1 2,258,271 - +1.4 2,258,303 + +1.3 2,258,544 + Rv2010 0.68 -0.6 2,258,545 - Rv2010 0.68 +0.0 2,258,545 - Rv2010 0.68 +0.2 2,258,738 - -0.5 2,258,747 + -1.2 2,258,790 - +0.1 2,259,229 + Rv2011c 0.87 +1.6 2,259,230 - Rv2011c 0.87 +2.0 2,259,230 - Rv2011c 0.87 -0.1 2,259,250 + +1.0 2,259,537 - Rv2012 0.43 -2.1 2,259,548 + Rv2012 0.45 -0.2 2,259,548 + Rv2012 0.45 -0.5 2,259,549 - Rv2012 0.45 +0.1 2,259,549 - Rv2012 0.45 -0.7 2,259,710 + Rv2012 0.78 -0.1 2,259,744 - Rv2012 0.84 -0.6 2,259,744 - Rv2012 0.84 -0.5 2,259,767 + Rv2012 0.89 +0.4 2,259,767 + Rv2012 0.89 -0.0 2,259,768 - Rv2012 0.89 +1.4 2,259,768 - Rv2012 0.89 -0.4 2,259,770 - Rv2012 0.90 -0.3 2,260,023 - +1.4 2,260,080 + +0.5 2,260,081 - +0.4 2,260,081 - -1.4 2,260,171 + +0.1 2,260,172 - -1.7
Or see this region's nucleotide sequence