Experiment: 7H9 with Dimethyl Sulfoxide 1 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0732 and Rv0733 overlap by 4 nucleotides Rv0733 and Rv0734 are separated by 2 nucleotides Rv0734 and Rv0735 are separated by 72 nucleotides Rv0735 and Rv0736 are separated by 63 nucleotides
Rv0732: Rv0732 - Probable preprotein translocase SecY, at 824,800 to 826,125
Rv0732
Rv0733: Rv0733 - Adenylate kinase Adk (ATP-AMP transphosphorylase), at 826,122 to 826,667
Rv0733
Rv0734: Rv0734 - Methionine aminopeptidase MapA (map) (peptidase M) (MetAP), at 826,670 to 827,470
Rv0734
Rv0735: Rv0735 - Probable alternative RNA polymerase sigma factor SigL, at 827,543 to 828,076
Rv0735
Rv0736: Rv0736 - Anti-sigma factor RslA, at 828,140 to 828,892
Rv0736
Position (kb) 826
827
828 Strain fitness (log2 ratio) -1
0
1
2 at 826.665 kb on + strand at 826.978 kb on - strand, within Rv0734 at 827.248 kb on + strand, within Rv0734 at 827.249 kb on - strand, within Rv0734 at 827.468 kb on + strand at 827.598 kb on - strand, within Rv0735 at 827.628 kb on - strand, within Rv0735 at 827.923 kb on + strand, within Rv0735 at 827.924 kb on - strand, within Rv0735 at 827.946 kb on - strand, within Rv0735 at 827.948 kb on + strand, within Rv0735 at 828.023 kb on + strand at 828.024 kb on - strand at 828.067 kb on + strand at 828.112 kb on + strand at 828.112 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Dimethyl Sulfoxide 1 vol% remove 826,665 + -1.1 826,978 - Rv0734 0.38 -1.0 827,248 + Rv0734 0.72 -0.2 827,249 - Rv0734 0.72 -1.2 827,468 + +2.0 827,598 - Rv0735 0.10 -0.3 827,628 - Rv0735 0.16 +0.1 827,923 + Rv0735 0.71 +0.2 827,924 - Rv0735 0.71 -0.3 827,946 - Rv0735 0.75 +0.3 827,948 + Rv0735 0.76 +0.4 828,023 + +0.2 828,024 - -0.0 828,067 + -0.5 828,112 + -1.2 828,112 + -1.0
Or see this region's nucleotide sequence