Experiment: 7H9 with Dimethyl Sulfoxide 1 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0427c and Rv0428c are separated by 2 nucleotides Rv0428c and Rv0429c overlap by 1 nucleotides Rv0429c and Rv0430 are separated by 336 nucleotides
Rv0427c: Rv0427c - Probable exodeoxyribonuclease III protein XthA (exonuclease III) (EXO III) (AP endonuclease VI), at 516,017 to 516,892
Rv0427c
Rv0428c: Rv0428c - GCN5-related N-acetyltransferase, at 516,895 to 517,803
Rv0428c
Rv0429c: Rv0429c - Probable polypeptide deformylase Def (PDF) (formylmethionine deformylase), at 517,803 to 518,396
Rv0429c
Rv0430: Rv0430 - Conserved hypothetical protein, at 518,733 to 519,041
Rv0430
Position (kb) 516
517
518 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 516.199 kb on - strand, within Rv0427c at 516.230 kb on + strand, within Rv0427c at 516.441 kb on + strand, within Rv0427c at 516.669 kb on + strand, within Rv0427c at 516.896 kb on + strand at 516.904 kb on - strand at 516.932 kb on + strand at 517.007 kb on + strand, within Rv0428c at 517.181 kb on + strand, within Rv0428c at 517.181 kb on + strand, within Rv0428c at 517.182 kb on - strand, within Rv0428c at 517.182 kb on - strand, within Rv0428c at 517.235 kb on - strand, within Rv0428c at 517.465 kb on - strand, within Rv0428c at 517.474 kb on + strand, within Rv0428c at 517.495 kb on + strand, within Rv0428c at 517.760 kb on + strand at 518.405 kb on - strand at 518.409 kb on + strand at 518.410 kb on - strand at 518.424 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Dimethyl Sulfoxide 1 vol% remove 516,199 - Rv0427c 0.21 -3.3 516,230 + Rv0427c 0.24 +0.6 516,441 + Rv0427c 0.48 -0.2 516,669 + Rv0427c 0.74 +0.7 516,896 + +0.8 516,904 - +0.5 516,932 + -0.2 517,007 + Rv0428c 0.12 +0.4 517,181 + Rv0428c 0.31 +1.1 517,181 + Rv0428c 0.31 +0.1 517,182 - Rv0428c 0.32 +1.2 517,182 - Rv0428c 0.32 +2.0 517,235 - Rv0428c 0.37 +0.4 517,465 - Rv0428c 0.63 -0.1 517,474 + Rv0428c 0.64 +1.3 517,495 + Rv0428c 0.66 -1.0 517,760 + -1.5 518,405 - +0.4 518,409 + -0.4 518,410 - -0.2 518,424 + +0.1
Or see this region's nucleotide sequence