Experiment: 7H9 with Dimethyl Sulfoxide 1 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0220 and Rv0221 are separated by 43 nucleotides Rv0221 and Rv0222 are separated by 30 nucleotides Rv0222 and Rv0223c are separated by 5 nucleotides
Rv0220: Rv0220 - Probable esterase LipC, at 262,812 to 264,023
Rv0220
Rv0221: Rv0221 - Possible triacylglycerol synthase (diacylglycerol acyltransferase), at 264,067 to 265,476
Rv0221
Rv0222: Rv0222 - Probable enoyl-CoA hydratase EchA1 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase), at 265,507 to 266,295
Rv0222
Rv0223c: Rv0223c - Probable aldehyde dehydrogenase, at 266,301 to 267,764
Rv0223c
Position (kb) 264
265
266 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 263.246 kb on - strand, within Rv0220 at 263.262 kb on - strand, within Rv0220 at 263.631 kb on + strand, within Rv0220 at 263.640 kb on + strand, within Rv0220 at 264.039 kb on - strand at 264.313 kb on + strand, within Rv0221 at 264.314 kb on - strand, within Rv0221 at 264.427 kb on + strand, within Rv0221 at 264.427 kb on + strand, within Rv0221 at 264.428 kb on - strand, within Rv0221 at 264.562 kb on + strand, within Rv0221 at 264.583 kb on - strand, within Rv0221 at 264.659 kb on - strand, within Rv0221 at 264.659 kb on - strand, within Rv0221 at 264.730 kb on - strand, within Rv0221 at 264.898 kb on + strand, within Rv0221 at 264.899 kb on - strand, within Rv0221 at 265.261 kb on + strand, within Rv0221 at 265.299 kb on + strand, within Rv0221 at 265.315 kb on + strand, within Rv0221 at 265.316 kb on - strand, within Rv0221 at 265.332 kb on + strand, within Rv0221 at 265.507 kb on - strand at 265.832 kb on - strand, within Rv0222 at 265.976 kb on - strand, within Rv0222 at 266.016 kb on - strand, within Rv0222 at 266.016 kb on - strand, within Rv0222 at 266.152 kb on - strand, within Rv0222 at 266.184 kb on + strand, within Rv0222 at 266.449 kb on + strand, within Rv0223c
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Dimethyl Sulfoxide 1 vol% remove 263,246 - Rv0220 0.36 +0.6 263,262 - Rv0220 0.37 -0.2 263,631 + Rv0220 0.68 -0.6 263,640 + Rv0220 0.68 +3.6 264,039 - -0.1 264,313 + Rv0221 0.17 -0.3 264,314 - Rv0221 0.18 -0.2 264,427 + Rv0221 0.26 +1.0 264,427 + Rv0221 0.26 -0.3 264,428 - Rv0221 0.26 -0.0 264,562 + Rv0221 0.35 -0.5 264,583 - Rv0221 0.37 +1.7 264,659 - Rv0221 0.42 -0.6 264,659 - Rv0221 0.42 +0.2 264,730 - Rv0221 0.47 +0.2 264,898 + Rv0221 0.59 +0.1 264,899 - Rv0221 0.59 +0.0 265,261 + Rv0221 0.85 +2.2 265,299 + Rv0221 0.87 +2.0 265,315 + Rv0221 0.89 -0.2 265,316 - Rv0221 0.89 -0.2 265,332 + Rv0221 0.90 -1.9 265,507 - -0.5 265,832 - Rv0222 0.41 +0.3 265,976 - Rv0222 0.59 -0.3 266,016 - Rv0222 0.65 +0.1 266,016 - Rv0222 0.65 +0.7 266,152 - Rv0222 0.82 -0.2 266,184 + Rv0222 0.86 -0.2 266,449 + Rv0223c 0.10 +0.8
Or see this region's nucleotide sequence