Experiment: 7H9 with Dimethyl Sulfoxide 1 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3340 and Rv3341 are separated by 11 nucleotides Rv3341 and Rv3342 overlap by 4 nucleotides Rv3342 and Rv3343c are separated by 8 nucleotides
Rv3340: Rv3340 - Probable O-acetylhomoserine sulfhydrylase MetC (homocysteine synthase) (O-acetylhomoserine (thiol)-lyase) (OAH sulfhydrylase) (O-acetyl-L-homoserine sulfhydrylase), at 3,726,127 to 3,727,476
Rv3340
Rv3341: Rv3341 - Probable homoserine O-acetyltransferase MetA (homoserine O-trans-acetylase) (homoserine transacetylase) (HTA), at 3,727,488 to 3,728,627
Rv3341
Rv3342: Rv3342 - Possible methyltransferase (methylase), at 3,728,624 to 3,729,355
Rv3342
Rv3343c: Rv3343c - PPE family protein PPE54, at 3,729,364 to 3,736,935
Rv3343c
Position (kb)
3727
3728
3729 Strain fitness (log2 ratio)
-1
0
1 at 3726.502 kb on + strand, within Rv3340 at 3727.111 kb on + strand, within Rv3340 at 3727.111 kb on + strand, within Rv3340 at 3727.111 kb on + strand, within Rv3340 at 3727.389 kb on + strand at 3727.504 kb on + strand at 3727.504 kb on + strand at 3728.699 kb on + strand, within Rv3342 at 3729.134 kb on - strand, within Rv3342 at 3729.212 kb on + strand, within Rv3342 at 3729.468 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Dimethyl Sulfoxide 1 vol% remove 3,726,502 + Rv3340 0.28 -0.7 3,727,111 + Rv3340 0.73 -0.9 3,727,111 + Rv3340 0.73 -0.7 3,727,111 + Rv3340 0.73 -0.6 3,727,389 + +0.3 3,727,504 + +0.0 3,727,504 + -1.0 3,728,699 + Rv3342 0.10 +0.1 3,729,134 - Rv3342 0.70 -0.6 3,729,212 + Rv3342 0.80 -0.2 3,729,468 - -0.4
Or see this region's nucleotide sequence