Experiment: 7H9 with Dimethyl Sulfoxide 1 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0050 and Rv0051 overlap by 4 nucleotides Rv0051 and Rv0052 are separated by 31 nucleotides Rv0052 and Rv0053 are separated by 218 nucleotides
Rv0050: Rv0050 - Probable bifunctional penicillin-binding protein 1A/1B PonA1 (murein polymerase) (PBP1): penicillin-insensitive transglycosylase (peptidoglycan TGASE) + penicillin-sensitive transpeptidase (DD-transpeptidase), at 53,663 to 55,699
Rv0050
Rv0051: Rv0051 - Probable conserved transmembrane protein, at 55,696 to 57,378
Rv0051
Rv0052: Rv0052 - Conserved protein, at 57,410 to 57,973
Rv0052
Rv0053: Rv0053 - 30S ribosomal protein S6 RpsF, at 58,192 to 58,482
Rv0053
Position (kb) 55
56
57
58 Strain fitness (log2 ratio) -2
-1
0
1 at 54.759 kb on + strand, within Rv0050 at 54.870 kb on - strand, within Rv0050 at 55.086 kb on - strand, within Rv0050 at 55.252 kb on - strand, within Rv0050 at 55.560 kb on + strand at 55.796 kb on + strand at 56.008 kb on + strand, within Rv0051 at 56.155 kb on + strand, within Rv0051 at 56.157 kb on + strand, within Rv0051 at 56.186 kb on - strand, within Rv0051 at 56.229 kb on - strand, within Rv0051 at 56.521 kb on + strand, within Rv0051 at 56.540 kb on - strand, within Rv0051 at 57.046 kb on + strand, within Rv0051 at 57.047 kb on - strand, within Rv0051 at 57.050 kb on + strand, within Rv0051 at 57.051 kb on - strand, within Rv0051 at 57.052 kb on + strand, within Rv0051 at 57.510 kb on + strand, within Rv0052 at 57.510 kb on + strand, within Rv0052 at 57.702 kb on + strand, within Rv0052 at 57.879 kb on - strand, within Rv0052 at 57.957 kb on - strand at 58.105 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Dimethyl Sulfoxide 1 vol% remove 54,759 + Rv0050 0.54 -1.7 54,870 - Rv0050 0.59 -1.1 55,086 - Rv0050 0.70 -0.5 55,252 - Rv0050 0.78 -1.6 55,560 + -1.0 55,796 + -2.0 56,008 + Rv0051 0.19 -0.6 56,155 + Rv0051 0.27 -0.4 56,157 + Rv0051 0.27 -1.5 56,186 - Rv0051 0.29 -0.8 56,229 - Rv0051 0.32 -0.8 56,521 + Rv0051 0.49 -0.1 56,540 - Rv0051 0.50 -0.7 57,046 + Rv0051 0.80 +0.2 57,047 - Rv0051 0.80 -0.5 57,050 + Rv0051 0.80 -0.6 57,051 - Rv0051 0.81 -1.2 57,052 + Rv0051 0.81 -0.6 57,510 + Rv0052 0.18 -0.1 57,510 + Rv0052 0.18 +0.1 57,702 + Rv0052 0.52 -0.3 57,879 - Rv0052 0.83 -1.8 57,957 - -0.1 58,105 + -0.4
Or see this region's nucleotide sequence