Experiment: 7H9 with Dimethyl Sulfoxide 1 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0032 and Rv0033 overlap by 4 nucleotides Rv0033 and Rv0034 overlap by 4 nucleotides Rv0034 and Rv0035 overlap by 4 nucleotides Rv0035 and Rv0036c are separated by 108 nucleotides Rv0036c and Rv0037c are separated by 47 nucleotides
Rv0032: Rv0032 - Possible 8-amino-7-oxononanoate synthase BioF2 (AONS) (8-amino-7-ketopelargonate synthase) (7-keto-8-amino-pelargonic acid synthetase) (7-KAP synthetase) (L-alanine--pimelyl CoA ligase), at 34,295 to 36,610
Rv0032
Rv0033: Rv0033 - Probable acyl carrier protein AcpA (ACP), at 36,607 to 36,870
Rv0033
Rv0034: Rv0034 - Conserved hypothetical protein, at 36,867 to 37,262
Rv0034
Rv0035: Rv0035 - Probable fatty-acid-CoA ligase FadD34 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase), at 37,259 to 38,947
Rv0035
Rv0036c: Rv0036c - Conserved protein, at 39,056 to 39,829
Rv0036c
Rv0037c: Rv0037c - Probable conserved integral membrane protein, at 39,877 to 41,202
Rv0037c
Position (kb) 37
38
39 Strain fitness (log2 ratio) -1
0
1
2
3 at 36.326 kb on + strand, within Rv0032 at 36.326 kb on + strand, within Rv0032 at 36.326 kb on + strand, within Rv0032 at 36.326 kb on + strand, within Rv0032 at 36.327 kb on - strand, within Rv0032 at 36.327 kb on - strand, within Rv0032 at 36.327 kb on - strand, within Rv0032 at 36.327 kb on - strand, within Rv0032 at 36.356 kb on + strand, within Rv0032 at 36.368 kb on + strand, within Rv0032 at 36.369 kb on - strand, within Rv0032 at 36.428 kb on + strand at 36.455 kb on + strand at 36.455 kb on + strand at 36.455 kb on + strand at 36.455 kb on + strand at 36.455 kb on + strand at 36.456 kb on - strand at 36.456 kb on - strand at 36.520 kb on - strand at 36.606 kb on + strand at 36.607 kb on - strand at 36.678 kb on - strand, within Rv0033 at 36.734 kb on + strand, within Rv0033 at 36.734 kb on + strand, within Rv0033 at 36.735 kb on - strand, within Rv0033 at 36.735 kb on - strand, within Rv0033 at 37.093 kb on + strand, within Rv0034 at 37.094 kb on - strand, within Rv0034 at 37.185 kb on - strand, within Rv0034 at 37.225 kb on - strand at 37.225 kb on - strand at 37.353 kb on + strand at 37.353 kb on + strand at 37.354 kb on - strand at 37.626 kb on + strand, within Rv0035 at 37.627 kb on - strand, within Rv0035 at 37.627 kb on - strand, within Rv0035 at 37.753 kb on - strand, within Rv0035 at 37.753 kb on - strand, within Rv0035 at 37.775 kb on + strand, within Rv0035 at 37.776 kb on - strand, within Rv0035 at 37.784 kb on + strand, within Rv0035 at 37.784 kb on + strand, within Rv0035 at 37.784 kb on + strand, within Rv0035 at 37.785 kb on - strand, within Rv0035 at 37.944 kb on + strand, within Rv0035 at 37.977 kb on + strand, within Rv0035 at 37.978 kb on - strand, within Rv0035 at 38.003 kb on + strand, within Rv0035 at 38.003 kb on + strand, within Rv0035 at 38.065 kb on + strand, within Rv0035 at 38.065 kb on + strand, within Rv0035 at 38.065 kb on + strand, within Rv0035 at 38.096 kb on + strand, within Rv0035 at 38.097 kb on - strand, within Rv0035 at 38.134 kb on - strand, within Rv0035 at 38.444 kb on + strand, within Rv0035 at 38.444 kb on + strand, within Rv0035 at 38.444 kb on + strand, within Rv0035 at 38.445 kb on - strand, within Rv0035 at 38.445 kb on - strand, within Rv0035 at 38.846 kb on + strand at 39.290 kb on + strand, within Rv0036c at 39.291 kb on - strand, within Rv0036c at 39.291 kb on - strand, within Rv0036c at 39.291 kb on - strand, within Rv0036c at 39.343 kb on + strand, within Rv0036c at 39.344 kb on - strand, within Rv0036c at 39.845 kb on + strand at 39.874 kb on - strand at 39.878 kb on + strand at 39.937 kb on + strand at 39.938 kb on - strand at 39.941 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Dimethyl Sulfoxide 1 vol% remove 36,326 + Rv0032 0.88 +0.2 36,326 + Rv0032 0.88 -0.4 36,326 + Rv0032 0.88 +0.4 36,326 + Rv0032 0.88 -1.2 36,327 - Rv0032 0.88 -0.8 36,327 - Rv0032 0.88 +0.2 36,327 - Rv0032 0.88 +1.2 36,327 - Rv0032 0.88 +0.1 36,356 + Rv0032 0.89 +0.0 36,368 + Rv0032 0.90 +1.7 36,369 - Rv0032 0.90 +0.1 36,428 + +0.7 36,455 + -0.1 36,455 + -0.4 36,455 + -0.1 36,455 + +0.3 36,455 + -0.1 36,456 - -0.1 36,456 - -0.7 36,520 - -1.0 36,606 + +1.3 36,607 - +1.2 36,678 - Rv0033 0.27 -0.3 36,734 + Rv0033 0.48 -0.7 36,734 + Rv0033 0.48 -1.4 36,735 - Rv0033 0.48 -0.3 36,735 - Rv0033 0.48 +0.3 37,093 + Rv0034 0.57 -0.0 37,094 - Rv0034 0.57 +0.5 37,185 - Rv0034 0.80 +0.5 37,225 - -0.7 37,225 - -0.3 37,353 + -0.2 37,353 + -0.4 37,354 - +0.8 37,626 + Rv0035 0.22 -1.4 37,627 - Rv0035 0.22 -1.0 37,627 - Rv0035 0.22 -0.2 37,753 - Rv0035 0.29 -0.1 37,753 - Rv0035 0.29 +0.6 37,775 + Rv0035 0.31 -0.1 37,776 - Rv0035 0.31 -0.7 37,784 + Rv0035 0.31 -0.6 37,784 + Rv0035 0.31 -0.4 37,784 + Rv0035 0.31 +0.2 37,785 - Rv0035 0.31 +0.2 37,944 + Rv0035 0.41 +2.8 37,977 + Rv0035 0.43 +2.3 37,978 - Rv0035 0.43 +0.5 38,003 + Rv0035 0.44 -0.9 38,003 + Rv0035 0.44 -0.4 38,065 + Rv0035 0.48 +0.1 38,065 + Rv0035 0.48 -0.3 38,065 + Rv0035 0.48 -0.3 38,096 + Rv0035 0.50 -0.4 38,097 - Rv0035 0.50 -0.4 38,134 - Rv0035 0.52 -0.3 38,444 + Rv0035 0.70 -0.8 38,444 + Rv0035 0.70 -0.3 38,444 + Rv0035 0.70 -0.3 38,445 - Rv0035 0.70 -0.5 38,445 - Rv0035 0.70 -0.1 38,846 + +1.0 39,290 + Rv0036c 0.30 -0.3 39,291 - Rv0036c 0.30 +0.1 39,291 - Rv0036c 0.30 -0.5 39,291 - Rv0036c 0.30 +0.8 39,343 + Rv0036c 0.37 +0.4 39,344 - Rv0036c 0.37 -0.6 39,845 + -0.1 39,874 - -0.6 39,878 + -0.4 39,937 + -1.0 39,938 - +0.8 39,941 + +0.6
Or see this region's nucleotide sequence