Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12510 and SGL_RS12515 overlap by 20 nucleotides SGL_RS12515 and SGL_RS12520 are separated by 200 nucleotides SGL_RS12520 and SGL_RS12525 are separated by 159 nucleotides SGL_RS12525 and SGL_RS12530 are separated by 203 nucleotides SGL_RS12530 and SGL_RS12535 are separated by 145 nucleotides
SGL_RS12510: SGL_RS12510 - lipoyl(octanoyl) transferase LipB, at 2,263,827 to 2,264,510
_RS12510
SGL_RS12515: SGL_RS12515 - hypothetical protein, at 2,264,491 to 2,264,781
_RS12515
SGL_RS12520: SGL_RS12520 - tRNA-Lys, at 2,264,982 to 2,265,053
_RS12520
SGL_RS12525: SGL_RS12525 - glycogen synthase GlgA, at 2,265,213 to 2,266,646
_RS12525
SGL_RS12530: SGL_RS12530 - DUF1830 domain-containing protein, at 2,266,850 to 2,267,344
_RS12530
SGL_RS12535: SGL_RS12535 - radical SAM protein, at 2,267,490 to 2,269,085
_RS12535
Position (kb)
2265
2266
2267 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1
2 at 2264.518 kb on + strand at 2264.540 kb on + strand, within SGL_RS12515 at 2264.801 kb on + strand at 2264.903 kb on - strand at 2264.920 kb on - strand at 2265.181 kb on - strand at 2265.251 kb on + strand at 2265.311 kb on - strand at 2265.324 kb on - strand at 2265.394 kb on + strand, within SGL_RS12525 at 2265.523 kb on + strand, within SGL_RS12525 at 2265.548 kb on - strand, within SGL_RS12525 at 2265.642 kb on + strand, within SGL_RS12525 at 2265.713 kb on + strand, within SGL_RS12525 at 2265.812 kb on + strand, within SGL_RS12525 at 2266.013 kb on + strand, within SGL_RS12525 at 2266.060 kb on - strand, within SGL_RS12525 at 2266.172 kb on + strand, within SGL_RS12525 at 2266.186 kb on + strand, within SGL_RS12525 at 2266.232 kb on + strand, within SGL_RS12525 at 2266.310 kb on - strand, within SGL_RS12525 at 2266.447 kb on - strand, within SGL_RS12525 at 2266.521 kb on + strand at 2266.540 kb on + strand at 2266.594 kb on + strand at 2266.792 kb on + strand at 2266.800 kb on - strand at 2266.897 kb on + strand at 2266.941 kb on + strand, within SGL_RS12530 at 2267.036 kb on + strand, within SGL_RS12530 at 2267.054 kb on + strand, within SGL_RS12530 at 2267.467 kb on + strand at 2267.469 kb on + strand at 2267.489 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 2,264,518 + -1.3 2,264,540 + SGL_RS12515 0.17 +1.0 2,264,801 + -2.6 2,264,903 - +0.1 2,264,920 - +0.4 2,265,181 - +0.5 2,265,251 + -0.9 2,265,311 - +2.6 2,265,324 - -1.5 2,265,394 + SGL_RS12525 0.13 -1.9 2,265,523 + SGL_RS12525 0.22 -0.9 2,265,548 - SGL_RS12525 0.23 -0.6 2,265,642 + SGL_RS12525 0.30 -1.2 2,265,713 + SGL_RS12525 0.35 -0.4 2,265,812 + SGL_RS12525 0.42 -0.8 2,266,013 + SGL_RS12525 0.56 -0.8 2,266,060 - SGL_RS12525 0.59 -0.3 2,266,172 + SGL_RS12525 0.67 -0.4 2,266,186 + SGL_RS12525 0.68 +0.9 2,266,232 + SGL_RS12525 0.71 -0.0 2,266,310 - SGL_RS12525 0.76 -1.0 2,266,447 - SGL_RS12525 0.86 -1.5 2,266,521 + -0.6 2,266,540 + +0.5 2,266,594 + -2.3 2,266,792 + +0.1 2,266,800 - +0.3 2,266,897 + -0.3 2,266,941 + SGL_RS12530 0.18 +0.6 2,267,036 + SGL_RS12530 0.38 +0.1 2,267,054 + SGL_RS12530 0.41 +0.0 2,267,467 + -5.4 2,267,469 + -6.2 2,267,489 - -6.9
Or see this region's nucleotide sequence