Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07305 and SGL_RS07310 are separated by 157 nucleotides SGL_RS07310 and SGL_RS07315 are separated by 69 nucleotides SGL_RS07315 and SGL_RS07320 are separated by 112 nucleotides SGL_RS07320 and SGL_RS07325 are separated by 324 nucleotides
SGL_RS07305: SGL_RS07305 - N-acetylmuramic acid 6-phosphate etherase, at 1,163,421 to 1,164,338
_RS07305
SGL_RS07310: SGL_RS07310 - DUF3110 domain-containing protein, at 1,164,496 to 1,165,017
_RS07310
SGL_RS07315: SGL_RS07315 - apocytochrome f, at 1,165,087 to 1,166,073
_RS07315
SGL_RS07320: SGL_RS07320 - cytochrome b6-f complex iron-sulfur subunit, at 1,166,186 to 1,166,728
_RS07320
SGL_RS07325: SGL_RS07325 - photosystem II reaction center protein PsbH, at 1,167,053 to 1,167,247
_RS07325
Position (kb)
1165
1166
1167 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 1164.112 kb on + strand, within SGL_RS07305 at 1164.705 kb on - strand, within SGL_RS07310 at 1164.769 kb on + strand, within SGL_RS07310 at 1164.787 kb on - strand, within SGL_RS07310 at 1164.815 kb on + strand, within SGL_RS07310 at 1164.847 kb on + strand, within SGL_RS07310 at 1164.948 kb on - strand, within SGL_RS07310 at 1164.958 kb on + strand, within SGL_RS07310 at 1164.963 kb on + strand, within SGL_RS07310 at 1165.034 kb on + strand at 1165.040 kb on + strand at 1165.042 kb on - strand at 1165.048 kb on - strand at 1167.068 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 1,164,112 + SGL_RS07305 0.75 -0.4 1,164,705 - SGL_RS07310 0.40 +0.8 1,164,769 + SGL_RS07310 0.52 +0.4 1,164,787 - SGL_RS07310 0.56 +1.3 1,164,815 + SGL_RS07310 0.61 +0.7 1,164,847 + SGL_RS07310 0.67 -0.1 1,164,948 - SGL_RS07310 0.87 +0.2 1,164,958 + SGL_RS07310 0.89 +0.1 1,164,963 + SGL_RS07310 0.89 -0.3 1,165,034 + +1.0 1,165,040 + +1.5 1,165,042 - +1.3 1,165,048 - +2.2 1,167,068 - -6.0
Or see this region's nucleotide sequence