Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03575 and SGL_RS19770 are separated by 163 nucleotides SGL_RS19770 and SGL_RS03580 are separated by 4 nucleotides SGL_RS03580 and SGL_RS03585 are separated by 56 nucleotides SGL_RS03585 and SGL_RS03590 overlap by 4 nucleotides
SGL_RS03575: SGL_RS03575 - Uma2 family endonuclease, at 358,760 to 359,350
_RS03575
SGL_RS19770: SGL_RS19770 - DUF4160 domain-containing protein, at 359,514 to 359,663
_RS19770
SGL_RS03580: SGL_RS03580 - DUF2442 domain-containing protein, at 359,668 to 359,973
_RS03580
SGL_RS03585: SGL_RS03585 - DegT/DnrJ/EryC1/StrS family aminotransferase, at 360,030 to 361,166
_RS03585
SGL_RS03590: SGL_RS03590 - ATP-grasp domain-containing protein, at 361,163 to 362,188
_RS03590
Position (kb)
360
361
362 Strain fitness (log2 ratio)
-8
-7
-6
-5
-4
-3
-2
-1
0
1 at 359.135 kb on - strand, within SGL_RS03575 at 359.477 kb on - strand at 360.007 kb on - strand at 360.074 kb on + strand at 360.203 kb on - strand, within SGL_RS03585 at 360.577 kb on + strand, within SGL_RS03585 at 360.585 kb on + strand, within SGL_RS03585 at 360.597 kb on + strand, within SGL_RS03585 at 360.630 kb on + strand, within SGL_RS03585 at 360.630 kb on + strand, within SGL_RS03585 at 360.653 kb on - strand, within SGL_RS03585 at 361.047 kb on - strand, within SGL_RS03585 at 361.603 kb on + strand, within SGL_RS03590 at 361.847 kb on + strand, within SGL_RS03590 at 361.889 kb on - strand, within SGL_RS03590 at 361.948 kb on + strand, within SGL_RS03590 at 362.055 kb on - strand, within SGL_RS03590
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 359,135 - SGL_RS03575 0.63 -0.3 359,477 - +0.1 360,007 - +0.2 360,074 + -6.3 360,203 - SGL_RS03585 0.15 -6.6 360,577 + SGL_RS03585 0.48 -7.9 360,585 + SGL_RS03585 0.49 -3.2 360,597 + SGL_RS03585 0.50 -5.6 360,630 + SGL_RS03585 0.53 -3.3 360,630 + SGL_RS03585 0.53 -5.9 360,653 - SGL_RS03585 0.55 -6.8 361,047 - SGL_RS03585 0.89 -6.3 361,603 + SGL_RS03590 0.43 -6.9 361,847 + SGL_RS03590 0.67 -6.5 361,889 - SGL_RS03590 0.71 -6.3 361,948 + SGL_RS03590 0.77 -5.5 362,055 - SGL_RS03590 0.87 -8.1
Or see this region's nucleotide sequence