Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02445 and SGL_RS02450 are separated by 80 nucleotides SGL_RS02450 and SGL_RS02455 overlap by 20 nucleotides SGL_RS02455 and SGL_RS02460 are separated by 76 nucleotides
SGL_RS02445: SGL_RS02445 - polyprenyl synthetase family protein, at 128,802 to 129,710
_RS02445
SGL_RS02450: SGL_RS02450 - hypothetical protein, at 129,791 to 130,186
_RS02450
SGL_RS02455: SGL_RS02455 - CPBP family intramembrane metalloprotease, at 130,167 to 131,039
_RS02455
SGL_RS02460: SGL_RS02460 - phosphate signaling complex protein PhoU, at 131,116 to 131,790
_RS02460
Position (kb) 129
130
131 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 129.609 kb on - strand, within SGL_RS02445 at 129.712 kb on - strand at 129.967 kb on - strand, within SGL_RS02450 at 130.005 kb on - strand, within SGL_RS02450 at 130.076 kb on + strand, within SGL_RS02450 at 130.076 kb on + strand, within SGL_RS02450 at 130.084 kb on - strand, within SGL_RS02450 at 130.130 kb on - strand, within SGL_RS02450 at 130.136 kb on - strand, within SGL_RS02450 at 131.057 kb on + strand at 131.067 kb on + strand at 131.165 kb on - strand at 131.173 kb on + strand at 131.181 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 129,609 - SGL_RS02445 0.89 -2.1 129,712 - +0.2 129,967 - SGL_RS02450 0.44 +3.5 130,005 - SGL_RS02450 0.54 +1.0 130,076 + SGL_RS02450 0.72 +1.4 130,076 + SGL_RS02450 0.72 +1.4 130,084 - SGL_RS02450 0.74 +1.2 130,130 - SGL_RS02450 0.86 +1.4 130,136 - SGL_RS02450 0.87 +1.5 131,057 + -0.1 131,067 + +0.2 131,165 - -0.5 131,173 + -0.3 131,181 - -4.1
Or see this region's nucleotide sequence