Experiment: low light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02995 and SGL_RS03000 are separated by 15 nucleotides SGL_RS03000 and SGL_RS03005 are separated by 74 nucleotides SGL_RS03005 and SGL_RS03010 are separated by 107 nucleotides
SGL_RS02995: SGL_RS02995 - Gfo/Idh/MocA family oxidoreductase, at 243,158 to 244,144
_RS02995
SGL_RS03000: SGL_RS03000 - spore maturation protein, at 244,160 to 244,708
_RS03000
SGL_RS03005: SGL_RS03005 - nucleoside recognition domain-containing protein, at 244,783 to 245,394
_RS03005
SGL_RS03010: SGL_RS03010 - 4-alpha-glucanotransferase, at 245,502 to 247,019
_RS03010
Position (kb) 244
245 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1 at 243.505 kb on - strand, within SGL_RS02995 at 243.709 kb on - strand, within SGL_RS02995 at 243.738 kb on + strand, within SGL_RS02995 at 243.741 kb on + strand, within SGL_RS02995 at 244.181 kb on + strand at 244.408 kb on + strand, within SGL_RS03000 at 244.417 kb on - strand, within SGL_RS03000 at 244.461 kb on + strand, within SGL_RS03000 at 244.540 kb on - strand, within SGL_RS03000 at 244.570 kb on - strand, within SGL_RS03000 at 245.163 kb on + strand, within SGL_RS03005 at 245.171 kb on - strand, within SGL_RS03005 at 245.406 kb on - strand at 245.426 kb on - strand at 245.468 kb on - strand at 245.601 kb on - strand at 245.620 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction low light; bubbling remove 243,505 - SGL_RS02995 0.35 -0.8 243,709 - SGL_RS02995 0.56 -2.6 243,738 + SGL_RS02995 0.59 -2.8 243,741 + SGL_RS02995 0.59 -2.8 244,181 + -2.5 244,408 + SGL_RS03000 0.45 -5.6 244,417 - SGL_RS03000 0.47 -5.1 244,461 + SGL_RS03000 0.55 -2.9 244,540 - SGL_RS03000 0.69 -0.8 244,570 - SGL_RS03000 0.75 -4.6 245,163 + SGL_RS03005 0.62 -6.9 245,171 - SGL_RS03005 0.63 -4.4 245,406 - -5.4 245,426 - -3.7 245,468 - -0.5 245,601 - -0.0 245,620 - -1.3
Or see this region's nucleotide sequence