Experiment: L-Glutamic Acid Monosodium Salt Monohydrate (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12985 and SGL_RS19465 are separated by 15 nucleotides SGL_RS19465 and SGL_RS12990 are separated by 30 nucleotides SGL_RS12990 and SGL_RS12995 overlap by 1 nucleotides SGL_RS12995 and SGL_RS13000 are separated by 348 nucleotides
SGL_RS12985: SGL_RS12985 - decarboxylating NADP(+)-dependent phosphogluconate dehydrogenase, at 2,387,424 to 2,388,851
_RS12985
SGL_RS19465: SGL_RS19465 - hypothetical protein, at 2,388,867 to 2,389,031
_RS19465
SGL_RS12990: SGL_RS12990 - hypothetical protein, at 2,389,062 to 2,389,481
_RS12990
SGL_RS12995: SGL_RS12995 - hypothetical protein, at 2,389,481 to 2,389,900
_RS12995
SGL_RS13000: SGL_RS13000 - SGNH/GDSL hydrolase family protein, at 2,390,249 to 2,391,454
_RS13000
Position (kb)
2389
2390 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2388.524 kb on - strand, within SGL_RS12985 at 2388.759 kb on - strand at 2388.875 kb on - strand at 2389.091 kb on - strand at 2389.124 kb on + strand, within SGL_RS12990 at 2389.425 kb on + strand, within SGL_RS12990 at 2389.565 kb on + strand, within SGL_RS12995 at 2389.586 kb on + strand, within SGL_RS12995 at 2389.609 kb on + strand, within SGL_RS12995 at 2389.715 kb on - strand, within SGL_RS12995 at 2390.155 kb on + strand at 2390.178 kb on - strand at 2390.194 kb on + strand at 2390.209 kb on - strand at 2390.373 kb on - strand, within SGL_RS13000 at 2390.441 kb on - strand, within SGL_RS13000 at 2390.520 kb on - strand, within SGL_RS13000 at 2390.665 kb on + strand, within SGL_RS13000 at 2390.693 kb on + strand, within SGL_RS13000 at 2390.703 kb on + strand, within SGL_RS13000 at 2390.716 kb on - strand, within SGL_RS13000 at 2390.779 kb on + strand, within SGL_RS13000 at 2390.784 kb on + strand, within SGL_RS13000 at 2390.784 kb on + strand, within SGL_RS13000
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamic Acid Monosodium Salt Monohydrate (N) remove 2,388,524 - SGL_RS12985 0.77 -4.1 2,388,759 - -1.7 2,388,875 - -0.3 2,389,091 - -0.6 2,389,124 + SGL_RS12990 0.15 +0.1 2,389,425 + SGL_RS12990 0.86 +1.0 2,389,565 + SGL_RS12995 0.20 +0.3 2,389,586 + SGL_RS12995 0.25 -0.0 2,389,609 + SGL_RS12995 0.30 +0.1 2,389,715 - SGL_RS12995 0.56 +0.3 2,390,155 + +0.5 2,390,178 - -0.2 2,390,194 + +0.5 2,390,209 - +0.3 2,390,373 - SGL_RS13000 0.10 -0.3 2,390,441 - SGL_RS13000 0.16 -0.0 2,390,520 - SGL_RS13000 0.22 -0.1 2,390,665 + SGL_RS13000 0.34 -0.2 2,390,693 + SGL_RS13000 0.37 +0.0 2,390,703 + SGL_RS13000 0.38 +0.1 2,390,716 - SGL_RS13000 0.39 +0.5 2,390,779 + SGL_RS13000 0.44 -0.1 2,390,784 + SGL_RS13000 0.44 -0.4 2,390,784 + SGL_RS13000 0.44 +0.2
Or see this region's nucleotide sequence