Experiment: L-Glutamic Acid Monosodium Salt Monohydrate (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04315 and SGL_RS04320 are separated by 22 nucleotides SGL_RS04320 and SGL_RS04325 are separated by 288 nucleotides SGL_RS04325 and SGL_RS04330 are separated by 139 nucleotides
SGL_RS04315: SGL_RS04315 - ABC transporter ATP-binding protein, at 523,016 to 523,798
_RS04315
SGL_RS04320: SGL_RS04320 - hypothetical protein, at 523,821 to 524,093
_RS04320
SGL_RS04325: SGL_RS04325 - CHAT domain-containing protein, at 524,382 to 529,595
_RS04325
SGL_RS04330: SGL_RS04330 - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase, at 529,735 to 530,688
_RS04330
Position (kb)
524
525
526
527
528
529
530 Strain fitness (log2 ratio)
-2
-1
0
1 at 523.397 kb on - strand, within SGL_RS04315 at 523.579 kb on + strand, within SGL_RS04315 at 523.615 kb on + strand, within SGL_RS04315 at 523.723 kb on + strand at 523.723 kb on + strand at 523.731 kb on - strand at 523.757 kb on + strand at 523.762 kb on + strand at 523.783 kb on - strand at 523.918 kb on - strand, within SGL_RS04320 at 523.941 kb on + strand, within SGL_RS04320 at 524.022 kb on + strand, within SGL_RS04320 at 524.317 kb on - strand at 524.459 kb on - strand at 524.480 kb on + strand at 524.599 kb on - strand at 524.715 kb on - strand at 524.850 kb on + strand at 524.937 kb on + strand, within SGL_RS04325 at 525.197 kb on - strand, within SGL_RS04325 at 525.200 kb on + strand, within SGL_RS04325 at 525.213 kb on - strand, within SGL_RS04325 at 525.215 kb on - strand, within SGL_RS04325 at 525.279 kb on - strand, within SGL_RS04325 at 525.425 kb on + strand, within SGL_RS04325 at 525.571 kb on - strand, within SGL_RS04325 at 525.571 kb on - strand, within SGL_RS04325 at 525.571 kb on - strand, within SGL_RS04325 at 525.683 kb on - strand, within SGL_RS04325 at 525.741 kb on - strand, within SGL_RS04325 at 525.767 kb on - strand, within SGL_RS04325 at 525.949 kb on - strand, within SGL_RS04325 at 525.949 kb on - strand, within SGL_RS04325 at 526.138 kb on - strand, within SGL_RS04325 at 526.177 kb on - strand, within SGL_RS04325 at 526.303 kb on + strand, within SGL_RS04325 at 526.511 kb on - strand, within SGL_RS04325 at 526.560 kb on - strand, within SGL_RS04325 at 526.584 kb on - strand, within SGL_RS04325 at 526.589 kb on - strand, within SGL_RS04325 at 526.672 kb on - strand, within SGL_RS04325 at 526.979 kb on - strand, within SGL_RS04325 at 526.987 kb on + strand, within SGL_RS04325 at 527.010 kb on + strand, within SGL_RS04325 at 527.073 kb on + strand, within SGL_RS04325 at 527.081 kb on + strand, within SGL_RS04325 at 527.218 kb on + strand, within SGL_RS04325 at 527.324 kb on + strand, within SGL_RS04325 at 527.584 kb on + strand, within SGL_RS04325 at 528.059 kb on - strand, within SGL_RS04325 at 528.375 kb on + strand, within SGL_RS04325 at 528.394 kb on - strand, within SGL_RS04325 at 528.622 kb on + strand, within SGL_RS04325 at 528.632 kb on - strand, within SGL_RS04325 at 528.829 kb on + strand, within SGL_RS04325 at 528.923 kb on + strand, within SGL_RS04325 at 529.067 kb on - strand, within SGL_RS04325 at 529.072 kb on - strand, within SGL_RS04325 at 529.194 kb on + strand at 529.263 kb on + strand at 529.340 kb on + strand at 529.391 kb on + strand at 529.397 kb on + strand at 529.399 kb on - strand at 529.400 kb on - strand at 529.405 kb on - strand at 529.406 kb on - strand at 529.423 kb on - strand at 529.425 kb on + strand at 529.428 kb on - strand at 529.438 kb on - strand at 529.521 kb on - strand at 529.567 kb on + strand at 529.575 kb on + strand at 529.581 kb on - strand at 529.583 kb on - strand at 529.761 kb on + strand at 530.141 kb on - strand, within SGL_RS04330 at 530.518 kb on - strand, within SGL_RS04330
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamic Acid Monosodium Salt Monohydrate (N) remove 523,397 - SGL_RS04315 0.49 +0.8 523,579 + SGL_RS04315 0.72 -0.8 523,615 + SGL_RS04315 0.77 -0.4 523,723 + -0.7 523,723 + -0.4 523,731 - -0.6 523,757 + -0.6 523,762 + -0.7 523,783 - +0.2 523,918 - SGL_RS04320 0.36 -0.2 523,941 + SGL_RS04320 0.44 -0.8 524,022 + SGL_RS04320 0.74 +0.2 524,317 - -0.0 524,459 - +0.3 524,480 + +0.8 524,599 - +0.6 524,715 - +0.4 524,850 + +0.4 524,937 + SGL_RS04325 0.11 +0.4 525,197 - SGL_RS04325 0.16 +0.5 525,200 + SGL_RS04325 0.16 +0.3 525,213 - SGL_RS04325 0.16 +0.1 525,215 - SGL_RS04325 0.16 -0.3 525,279 - SGL_RS04325 0.17 +0.7 525,425 + SGL_RS04325 0.20 -0.3 525,571 - SGL_RS04325 0.23 +0.4 525,571 - SGL_RS04325 0.23 +0.8 525,571 - SGL_RS04325 0.23 +0.6 525,683 - SGL_RS04325 0.25 +0.2 525,741 - SGL_RS04325 0.26 +0.7 525,767 - SGL_RS04325 0.27 +0.2 525,949 - SGL_RS04325 0.30 +0.1 525,949 - SGL_RS04325 0.30 +0.7 526,138 - SGL_RS04325 0.34 +0.1 526,177 - SGL_RS04325 0.34 -1.6 526,303 + SGL_RS04325 0.37 +0.1 526,511 - SGL_RS04325 0.41 +0.4 526,560 - SGL_RS04325 0.42 +0.2 526,584 - SGL_RS04325 0.42 +0.7 526,589 - SGL_RS04325 0.42 +0.7 526,672 - SGL_RS04325 0.44 +0.4 526,979 - SGL_RS04325 0.50 +0.8 526,987 + SGL_RS04325 0.50 -0.1 527,010 + SGL_RS04325 0.50 +1.0 527,073 + SGL_RS04325 0.52 +0.5 527,081 + SGL_RS04325 0.52 +0.3 527,218 + SGL_RS04325 0.54 -0.9 527,324 + SGL_RS04325 0.56 +0.1 527,584 + SGL_RS04325 0.61 +0.1 528,059 - SGL_RS04325 0.71 +0.4 528,375 + SGL_RS04325 0.77 +0.2 528,394 - SGL_RS04325 0.77 -1.0 528,622 + SGL_RS04325 0.81 +0.1 528,632 - SGL_RS04325 0.82 +0.4 528,829 + SGL_RS04325 0.85 +1.0 528,923 + SGL_RS04325 0.87 +1.3 529,067 - SGL_RS04325 0.90 +0.2 529,072 - SGL_RS04325 0.90 -0.3 529,194 + +0.3 529,263 + +0.3 529,340 + -0.4 529,391 + +0.3 529,397 + -0.2 529,399 - +0.4 529,400 - +0.4 529,405 - -0.1 529,406 - +0.1 529,423 - -0.8 529,425 + +0.4 529,428 - +0.4 529,438 - -0.1 529,521 - +0.2 529,567 + +0.3 529,575 + +0.7 529,581 - +0.8 529,583 - -0.2 529,761 + -2.0 530,141 - SGL_RS04330 0.43 -1.7 530,518 - SGL_RS04330 0.82 -2.6
Or see this region's nucleotide sequence