Experiment: L-Proline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS06460 and SGL_RS06465 are separated by 3 nucleotides SGL_RS06465 and SGL_RS06470 are separated by 47 nucleotides SGL_RS06470 and SGL_RS06475 are separated by 108 nucleotides SGL_RS06475 and SGL_RS06480 are separated by 263 nucleotides
SGL_RS06460: SGL_RS06460 - fatty acid desaturase, at 981,691 to 982,629
_RS06460
SGL_RS06465: SGL_RS06465 - hypothetical protein, at 982,633 to 982,920
_RS06465
SGL_RS06470: SGL_RS06470 - chlorophyll a/b-binding protein, at 982,968 to 983,180
_RS06470
SGL_RS06475: SGL_RS06475 - hypothetical protein, at 983,289 to 983,540
_RS06475
SGL_RS06480: SGL_RS06480 - sigma-70 family RNA polymerase sigma factor, at 983,804 to 984,475
_RS06480
Position (kb) 983
984 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 982.453 kb on - strand, within SGL_RS06460 at 982.495 kb on + strand, within SGL_RS06460 at 982.626 kb on - strand at 982.990 kb on - strand, within SGL_RS06470 at 982.997 kb on + strand, within SGL_RS06470 at 983.034 kb on + strand, within SGL_RS06470 at 983.147 kb on + strand, within SGL_RS06470 at 983.211 kb on + strand at 983.219 kb on - strand at 983.219 kb on - strand at 983.335 kb on + strand, within SGL_RS06475 at 983.337 kb on + strand, within SGL_RS06475 at 983.390 kb on - strand, within SGL_RS06475 at 983.441 kb on + strand, within SGL_RS06475 at 983.474 kb on - strand, within SGL_RS06475 at 983.603 kb on + strand at 983.611 kb on - strand at 983.701 kb on + strand at 984.165 kb on + strand, within SGL_RS06480 at 984.365 kb on - strand, within SGL_RS06480 at 984.430 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (N) remove 982,453 - SGL_RS06460 0.81 -3.6 982,495 + SGL_RS06460 0.86 -3.0 982,626 - -1.8 982,990 - SGL_RS06470 0.10 +2.7 982,997 + SGL_RS06470 0.14 +0.2 983,034 + SGL_RS06470 0.31 -0.5 983,147 + SGL_RS06470 0.84 -0.2 983,211 + +0.0 983,219 - -0.5 983,219 - +0.2 983,335 + SGL_RS06475 0.18 +0.2 983,337 + SGL_RS06475 0.19 +0.3 983,390 - SGL_RS06475 0.40 -0.3 983,441 + SGL_RS06475 0.60 +0.7 983,474 - SGL_RS06475 0.73 +0.8 983,603 + +0.6 983,611 - -0.1 983,701 + -0.9 984,165 + SGL_RS06480 0.54 -1.6 984,365 - SGL_RS06480 0.83 -1.3 984,430 - -1.3
Or see this region's nucleotide sequence