Experiment: nitrate (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07690 and SGL_RS19585 are separated by 150 nucleotides SGL_RS19585 and SGL_RS07695 are separated by 125 nucleotides SGL_RS07695 and SGL_RS07700 are separated by 85 nucleotides
SGL_RS07690: SGL_RS07690 - tryptophan--tRNA ligase, at 1,243,274 to 1,244,287
_RS07690
SGL_RS19585: SGL_RS19585 - hypothetical protein, at 1,244,438 to 1,244,605
_RS19585
SGL_RS07695: SGL_RS07695 - bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK, at 1,244,731 to 1,245,309
_RS07695
SGL_RS07700: SGL_RS07700 - ribosome silencing factor, at 1,245,395 to 1,245,859
_RS07700
Position (kb) 1244
1245 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 1244.283 kb on + strand at 1244.335 kb on - strand at 1244.414 kb on - strand at 1244.552 kb on + strand, within SGL_RS19585 at 1244.621 kb on + strand at 1244.628 kb on - strand at 1244.835 kb on - strand, within SGL_RS07695 at 1244.882 kb on - strand, within SGL_RS07695 at 1244.989 kb on - strand, within SGL_RS07695 at 1245.080 kb on - strand, within SGL_RS07695 at 1245.094 kb on + strand, within SGL_RS07695 at 1245.125 kb on + strand, within SGL_RS07695 at 1245.141 kb on + strand, within SGL_RS07695 at 1245.148 kb on + strand, within SGL_RS07695 at 1245.207 kb on + strand, within SGL_RS07695 at 1245.339 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate (N) remove 1,244,283 + -0.0 1,244,335 - -5.9 1,244,414 - -5.5 1,244,552 + SGL_RS19585 0.68 -5.4 1,244,621 + +0.1 1,244,628 - +0.2 1,244,835 - SGL_RS07695 0.18 +0.0 1,244,882 - SGL_RS07695 0.26 +0.4 1,244,989 - SGL_RS07695 0.45 +0.7 1,245,080 - SGL_RS07695 0.60 +0.4 1,245,094 + SGL_RS07695 0.63 +0.0 1,245,125 + SGL_RS07695 0.68 -0.4 1,245,141 + SGL_RS07695 0.71 -0.1 1,245,148 + SGL_RS07695 0.72 -0.0 1,245,207 + SGL_RS07695 0.82 +0.4 1,245,339 + +0.3
Or see this region's nucleotide sequence