Experiment: nitrate (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS17220 and SGL_RS17225 are separated by 68 nucleotides SGL_RS17225 and SGL_RS17230 are separated by 23 nucleotides SGL_RS17230 and SGL_RS17235 are separated by 107 nucleotides SGL_RS17235 and SGL_RS17240 are separated by 95 nucleotides
SGL_RS17220: SGL_RS17220 - DUF4346 domain-containing protein, at 3,318,829 to 3,319,212
_RS17220
SGL_RS17225: SGL_RS17225 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 3,319,281 to 3,320,051
_RS17225
SGL_RS17230: SGL_RS17230 - L-aspartate oxidase, at 3,320,075 to 3,321,736
_RS17230
SGL_RS17235: SGL_RS17235 - hypothetical protein, at 3,321,844 to 3,322,281
_RS17235
SGL_RS17240: SGL_RS17240 - photosystem I reaction center subunit PsaK, at 3,322,377 to 3,322,649
_RS17240
Position (kb)
3320
3321
3322 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 3319.494 kb on + strand, within SGL_RS17225 at 3319.803 kb on - strand, within SGL_RS17225 at 3320.232 kb on - strand at 3320.239 kb on + strand at 3320.261 kb on - strand, within SGL_RS17230 at 3320.520 kb on + strand, within SGL_RS17230 at 3320.545 kb on - strand, within SGL_RS17230 at 3321.006 kb on - strand, within SGL_RS17230 at 3321.085 kb on - strand, within SGL_RS17230 at 3321.184 kb on - strand, within SGL_RS17230 at 3321.253 kb on - strand, within SGL_RS17230 at 3321.382 kb on + strand, within SGL_RS17230 at 3321.590 kb on + strand at 3321.678 kb on + strand at 3321.685 kb on - strand at 3321.860 kb on - strand at 3322.099 kb on + strand, within SGL_RS17235 at 3322.123 kb on + strand, within SGL_RS17235 at 3322.400 kb on + strand at 3322.494 kb on - strand, within SGL_RS17240 at 3322.494 kb on - strand, within SGL_RS17240 at 3322.503 kb on + strand, within SGL_RS17240 at 3322.503 kb on + strand, within SGL_RS17240 at 3322.512 kb on - strand, within SGL_RS17240 at 3322.604 kb on - strand, within SGL_RS17240 at 3322.612 kb on + strand, within SGL_RS17240
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate (N) remove 3,319,494 + SGL_RS17225 0.28 -3.4 3,319,803 - SGL_RS17225 0.68 -3.6 3,320,232 - -2.0 3,320,239 + -4.1 3,320,261 - SGL_RS17230 0.11 -3.6 3,320,520 + SGL_RS17230 0.27 -4.3 3,320,545 - SGL_RS17230 0.28 -3.6 3,321,006 - SGL_RS17230 0.56 -5.1 3,321,085 - SGL_RS17230 0.61 -5.1 3,321,184 - SGL_RS17230 0.67 -5.9 3,321,253 - SGL_RS17230 0.71 -3.8 3,321,382 + SGL_RS17230 0.79 -4.9 3,321,590 + -1.1 3,321,678 + -4.2 3,321,685 - -5.7 3,321,860 - +0.1 3,322,099 + SGL_RS17235 0.58 -0.1 3,322,123 + SGL_RS17235 0.64 -0.0 3,322,400 + -0.3 3,322,494 - SGL_RS17240 0.43 -0.1 3,322,494 - SGL_RS17240 0.43 -0.5 3,322,503 + SGL_RS17240 0.46 -0.4 3,322,503 + SGL_RS17240 0.46 -0.2 3,322,512 - SGL_RS17240 0.49 -1.0 3,322,604 - SGL_RS17240 0.83 -0.5 3,322,612 + SGL_RS17240 0.86 -1.1
Or see this region's nucleotide sequence