Experiment: nitrate (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14575 and SGL_RS14580 are separated by 222 nucleotides SGL_RS14580 and SGL_RS14585 are separated by 55 nucleotides SGL_RS14585 and SGL_RS14590 are separated by 56 nucleotides SGL_RS14590 and SGL_RS14595 overlap by 13 nucleotides
SGL_RS14575: SGL_RS14575 - RNA-binding protein, at 2,724,648 to 2,725,103
_RS14575
SGL_RS14580: SGL_RS14580 - ribose-5-phosphate isomerase RpiA, at 2,725,326 to 2,726,033
_RS14580
SGL_RS14585: SGL_RS14585 - TolB family protein, at 2,726,089 to 2,726,640
_RS14585
SGL_RS14590: SGL_RS14590 - hypothetical protein, at 2,726,697 to 2,727,059
_RS14590
SGL_RS14595: SGL_RS14595 - phospholipase D-like domain-containing protein, at 2,727,047 to 2,728,708
_RS14595
Position (kb)
2726
2727 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2725.129 kb on - strand at 2725.529 kb on - strand, within SGL_RS14580 at 2725.652 kb on - strand, within SGL_RS14580 at 2726.060 kb on - strand at 2726.241 kb on - strand, within SGL_RS14585 at 2726.383 kb on - strand, within SGL_RS14585 at 2726.837 kb on - strand, within SGL_RS14590 at 2726.959 kb on + strand, within SGL_RS14590 at 2727.098 kb on + strand at 2727.106 kb on - strand at 2727.151 kb on + strand at 2727.156 kb on + strand at 2727.175 kb on - strand at 2727.184 kb on + strand at 2727.192 kb on - strand at 2727.374 kb on + strand, within SGL_RS14595 at 2727.457 kb on - strand, within SGL_RS14595 at 2727.484 kb on + strand, within SGL_RS14595 at 2727.497 kb on + strand, within SGL_RS14595 at 2727.549 kb on + strand, within SGL_RS14595 at 2727.588 kb on + strand, within SGL_RS14595 at 2727.640 kb on - strand, within SGL_RS14595
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate (N) remove 2,725,129 - +0.2 2,725,529 - SGL_RS14580 0.29 -2.7 2,725,652 - SGL_RS14580 0.46 -4.1 2,726,060 - +0.2 2,726,241 - SGL_RS14585 0.28 -4.3 2,726,383 - SGL_RS14585 0.53 -4.2 2,726,837 - SGL_RS14590 0.39 +0.4 2,726,959 + SGL_RS14590 0.72 +0.3 2,727,098 + +0.2 2,727,106 - +0.1 2,727,151 + -0.1 2,727,156 + +0.4 2,727,175 - +0.3 2,727,184 + +0.6 2,727,192 - +0.2 2,727,374 + SGL_RS14595 0.20 +0.1 2,727,457 - SGL_RS14595 0.25 -0.2 2,727,484 + SGL_RS14595 0.26 +0.3 2,727,497 + SGL_RS14595 0.27 +0.0 2,727,549 + SGL_RS14595 0.30 -0.3 2,727,588 + SGL_RS14595 0.33 +0.3 2,727,640 - SGL_RS14595 0.36 -0.0
Or see this region's nucleotide sequence