Experiment: nitrate (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS08700 and SGL_RS08705 are separated by 198 nucleotides SGL_RS08705 and SGL_RS08710 are separated by 248 nucleotides SGL_RS08710 and SGL_RS08715 are separated by 34 nucleotides
SGL_RS08700: SGL_RS08700 - cation-transporting P-type ATPase, at 1,457,213 to 1,459,930
_RS08700
SGL_RS08705: SGL_RS08705 - hypothetical protein, at 1,460,129 to 1,460,566
_RS08705
SGL_RS08710: SGL_RS08710 - folylpolyglutamate synthase/dihydrofolate synthase family protein, at 1,460,815 to 1,462,101
_RS08710
SGL_RS08715: SGL_RS08715 - fatty acid desaturase, at 1,462,136 to 1,463,245
_RS08715
Position (kb)
1460
1461
1462
1463 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1460.536 kb on + strand at 1460.550 kb on + strand at 1460.550 kb on + strand at 1460.784 kb on + strand at 1461.066 kb on - strand, within SGL_RS08710 at 1461.092 kb on + strand, within SGL_RS08710 at 1461.130 kb on + strand, within SGL_RS08710 at 1461.231 kb on + strand, within SGL_RS08710 at 1461.351 kb on - strand, within SGL_RS08710 at 1461.393 kb on + strand, within SGL_RS08710 at 1461.421 kb on - strand, within SGL_RS08710 at 1461.541 kb on + strand, within SGL_RS08710 at 1461.549 kb on - strand, within SGL_RS08710 at 1461.598 kb on - strand, within SGL_RS08710 at 1461.717 kb on - strand, within SGL_RS08710 at 1461.942 kb on + strand, within SGL_RS08710 at 1461.954 kb on - strand, within SGL_RS08710 at 1462.021 kb on + strand at 1462.067 kb on + strand at 1462.210 kb on - strand at 1462.319 kb on - strand, within SGL_RS08715 at 1462.320 kb on - strand, within SGL_RS08715 at 1462.733 kb on + strand, within SGL_RS08715 at 1462.753 kb on - strand, within SGL_RS08715 at 1462.993 kb on + strand, within SGL_RS08715 at 1463.091 kb on + strand, within SGL_RS08715
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate (N) remove 1,460,536 + -0.5 1,460,550 + +0.1 1,460,550 + +0.2 1,460,784 + -0.0 1,461,066 - SGL_RS08710 0.20 -3.3 1,461,092 + SGL_RS08710 0.22 -2.8 1,461,130 + SGL_RS08710 0.24 -2.8 1,461,231 + SGL_RS08710 0.32 -4.8 1,461,351 - SGL_RS08710 0.42 -3.5 1,461,393 + SGL_RS08710 0.45 -3.3 1,461,421 - SGL_RS08710 0.47 -3.3 1,461,541 + SGL_RS08710 0.56 -3.7 1,461,549 - SGL_RS08710 0.57 -5.0 1,461,598 - SGL_RS08710 0.61 -4.6 1,461,717 - SGL_RS08710 0.70 -2.8 1,461,942 + SGL_RS08710 0.88 -1.5 1,461,954 - SGL_RS08710 0.89 -4.1 1,462,021 + -3.3 1,462,067 + -4.0 1,462,210 - -0.5 1,462,319 - SGL_RS08715 0.16 -0.3 1,462,320 - SGL_RS08715 0.17 +0.2 1,462,733 + SGL_RS08715 0.54 -0.2 1,462,753 - SGL_RS08715 0.56 -0.1 1,462,993 + SGL_RS08715 0.77 +0.5 1,463,091 + SGL_RS08715 0.86 -0.0
Or see this region's nucleotide sequence