Experiment: nitrite (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14525 and SGL_RS14530 are separated by 65 nucleotides SGL_RS14530 and SGL_RS14535 are separated by 118 nucleotides SGL_RS14535 and SGL_RS14540 are separated by 13 nucleotides
SGL_RS14525: SGL_RS14525 - reverse transcriptase domain-containing protein, at 2,711,740 to 2,713,305
_RS14525
SGL_RS14530: SGL_RS14530 - carbamoyl-phosphate synthase large subunit, at 2,713,371 to 2,716,616
_RS14530
SGL_RS14535: SGL_RS14535 - hypothetical protein, at 2,716,735 to 2,717,163
_RS14535
SGL_RS14540: SGL_RS14540 - bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR, at 2,717,177 to 2,717,713
_RS14540
Position (kb)
2713
2714
2715
2716
2717 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2713.325 kb on + strand at 2713.325 kb on - strand at 2713.344 kb on + strand at 2713.352 kb on - strand at 2713.373 kb on + strand at 2713.408 kb on - strand at 2714.496 kb on - strand, within SGL_RS14530 at 2716.663 kb on + strand at 2716.712 kb on - strand at 2716.759 kb on + strand at 2716.767 kb on - strand at 2716.767 kb on - strand at 2716.821 kb on + strand, within SGL_RS14535 at 2717.058 kb on - strand, within SGL_RS14535 at 2717.498 kb on - strand, within SGL_RS14540 at 2717.583 kb on - strand, within SGL_RS14540 at 2717.608 kb on - strand, within SGL_RS14540
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite (N) remove 2,713,325 + -0.3 2,713,325 - +0.2 2,713,344 + -0.0 2,713,352 - +0.1 2,713,373 + +2.4 2,713,408 - -2.2 2,714,496 - SGL_RS14530 0.35 -0.2 2,716,663 + +0.3 2,716,712 - -1.6 2,716,759 + -0.3 2,716,767 - +0.2 2,716,767 - -0.3 2,716,821 + SGL_RS14535 0.20 +0.4 2,717,058 - SGL_RS14535 0.75 +0.4 2,717,498 - SGL_RS14540 0.60 +2.4 2,717,583 - SGL_RS14540 0.76 +0.0 2,717,608 - SGL_RS14540 0.80 +0.8
Or see this region's nucleotide sequence