Experiment: nitrite (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS10795 and SGL_RS10800 are separated by 131 nucleotides SGL_RS10800 and SGL_RS10805 overlap by 14 nucleotides SGL_RS10805 and SGL_RS10810 are separated by 93 nucleotides SGL_RS10810 and SGL_RS10815 overlap by 23 nucleotides SGL_RS10815 and SGL_RS10820 are separated by 92 nucleotides
SGL_RS10795: SGL_RS10795 - pentapeptide repeat-containing protein, at 1,888,778 to 1,889,740
_RS10795
SGL_RS10800: SGL_RS10800 - 3-isopropylmalate dehydratase small subunit, at 1,889,872 to 1,890,474
_RS10800
SGL_RS10805: SGL_RS10805 - CTP synthase (glutamine hydrolyzing), at 1,890,461 to 1,892,119
_RS10805
SGL_RS10810: SGL_RS10810 - DUF4926 domain-containing protein, at 1,892,213 to 1,892,500
_RS10810
SGL_RS10815: SGL_RS10815 - hypothetical protein, at 1,892,478 to 1,892,825
_RS10815
SGL_RS10820: SGL_RS10820 - sigma 54-interacting transcriptional regulator, at 1,892,918 to 1,895,425
_RS10820
Position (kb)
1890
1891
1892
1893 Strain fitness (log2 ratio)
-1
0
1 at 1889.779 kb on + strand at 1890.495 kb on - strand at 1892.280 kb on + strand, within SGL_RS10810 at 1892.288 kb on - strand, within SGL_RS10810 at 1892.451 kb on - strand, within SGL_RS10810 at 1892.511 kb on + strand at 1892.530 kb on - strand, within SGL_RS10815 at 1892.753 kb on + strand, within SGL_RS10815 at 1892.966 kb on + strand at 1892.966 kb on + strand at 1893.045 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite (N) remove 1,889,779 + +0.3 1,890,495 - -0.3 1,892,280 + SGL_RS10810 0.23 +0.6 1,892,288 - SGL_RS10810 0.26 +0.8 1,892,451 - SGL_RS10810 0.83 +0.7 1,892,511 + -0.3 1,892,530 - SGL_RS10815 0.15 -0.0 1,892,753 + SGL_RS10815 0.79 +0.3 1,892,966 + +0.3 1,892,966 + -0.8 1,893,045 - -0.2
Or see this region's nucleotide sequence