Experiment: nitrite (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS10360 and SGL_RS10365 are separated by 69 nucleotides SGL_RS10365 and SGL_RS10370 overlap by 97 nucleotides SGL_RS10370 and SGL_RS10375 are separated by 5 nucleotides SGL_RS10375 and SGL_RS10380 are separated by 61 nucleotides
SGL_RS10360: SGL_RS10360 - hypothetical protein, at 1,798,804 to 1,799,538
_RS10360
SGL_RS10365: SGL_RS10365 - CbiQ family ECF transporter T component, at 1,799,608 to 1,800,483
_RS10365
SGL_RS10370: SGL_RS10370 - anthranilate synthase component I, at 1,800,387 to 1,801,844
_RS10370
SGL_RS10375: SGL_RS10375 - EI24 domain-containing protein, at 1,801,850 to 1,802,680
_RS10375
SGL_RS10380: SGL_RS10380 - oxygen-independent coproporphyrinogen III oxidase, at 1,802,742 to 1,804,142
_RS10380
Position (kb)
1800
1801
1802 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1799.423 kb on - strand, within SGL_RS10360 at 1799.476 kb on + strand at 1799.481 kb on + strand at 1799.491 kb on - strand at 1799.613 kb on - strand at 1801.049 kb on + strand, within SGL_RS10370 at 1801.119 kb on - strand, within SGL_RS10370 at 1801.309 kb on + strand, within SGL_RS10370 at 1801.428 kb on + strand, within SGL_RS10370 at 1801.486 kb on - strand, within SGL_RS10370 at 1801.677 kb on - strand, within SGL_RS10370 at 1801.731 kb on - strand at 1802.075 kb on + strand, within SGL_RS10375 at 1802.496 kb on + strand, within SGL_RS10375 at 1802.843 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite (N) remove 1,799,423 - SGL_RS10360 0.84 +1.5 1,799,476 + +0.2 1,799,481 + -0.7 1,799,491 - +0.6 1,799,613 - -4.6 1,801,049 + SGL_RS10370 0.45 -2.1 1,801,119 - SGL_RS10370 0.50 -3.8 1,801,309 + SGL_RS10370 0.63 -2.9 1,801,428 + SGL_RS10370 0.71 -5.7 1,801,486 - SGL_RS10370 0.75 -5.6 1,801,677 - SGL_RS10370 0.88 -4.5 1,801,731 - -3.1 1,802,075 + SGL_RS10375 0.27 +0.4 1,802,496 + SGL_RS10375 0.78 -0.3 1,802,843 + +0.3
Or see this region's nucleotide sequence